环氧合酶-2抑制剂对非小细胞肺癌A549细胞VEGF表达的影响  

Effect of inhibitor of cyclooxygenase-2 on the expression of VEGF in A549 cell lines of non-sman cell lung cancers

作　　者：童伟[1] 刘永煜[1] 玉寒冰[1] 罗全[1] 于占武[1] 赵建[1] 

机构地区：[1]辽宁省肿瘤医院胸外科

出　　处：《解剖学研究》2015年第6期454-456,467,共4页Anatomy Research

摘　　要：目的探讨环氧合酶-2抑制剂对非小细胞肺癌A549细胞血管内皮生长因子（VEGF）表达的影响。方法加不同浓度塞来昔布与A549肺癌细胞共培养，MTT法检测细胞增殖，501xmol／L塞来昔布作用于A549细胞48h，RT—PCR方法检测各组细胞的VEGF基因表达水平，Westernblot方法检测各组细胞的VEGF蛋白表达。结果塞来昔布在体外对细胞株A549的生长抑制作用呈剂量、时间依赖性；各组细胞培养48h后，相比于对照组，RT-PCR方法检测发现塞来昔布组细胞VEGFmRNA表达显著降低（P〈0．01），Westernblot方法检测发现塞来昔布组细胞VEGF蛋白表达显著降低（P〈0．01）。结论塞来昔布可能通过调节VEGF的表达抑制A549细胞增殖。Objective To study the effect of inhibitor of cyclooxygenase-2 on the expression of vascular endothelial growth factor （VEGF） in A549 cell lines of non-sman cell lung cancers. Methods The proliferation of A549 lung carcinoma cells was measured by MTY assay After A549 cells were treated with different dose celecoxib; After A549 cells were treated with celecoxib 50 μmol/L for 48 h, the level of VEGF mRNA was detected RT-PCR, and the expressions of VEGF protein was detected by western blot method. Results Celecoxib could inhibit the proliferation of A549 cell by a time-and dose-dependent manner. Compared with control group, the level of VEGF mRNA was decreased significantly detected by RT-PCR after A549 cells were trealed with celecoxib 50 μmol·L-1 for 48 h, P〈0.01 and the expression of VEGF protein was decreased significantly detected by western blot method, P〈0.01. Conclusion celccoxib might inhibite the proliferation of A549 lung carcinoma ceils by regulating the expressions of VEGF.

关 键 词：非小细胞肺癌 环氧合酶-2 塞来昔布 血管内皮生长因子 A549细胞 

分 类 号：R734.2[医药卫生—肿瘤]