地衣芽孢杆菌降解茶皂素的研究  

Study on the degradation of tea saponin by Bacillus licheniformis

作　　者：黄浦[1,2] 肖瑜[1,2] 覃妍[1,2] 范文哲[1,2] 

机构地区：[1]桂林理工大学广西环境污染控制理论与技术重点实验室,广西桂林541004 [2]桂林理工大学广西岩溶地区水污染控制与用水安全保障协同创新中心,广西桂林541004

出　　处：《安徽大学学报（自然科学版）》2016年第1期91-97,共7页Journal of Anhui University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(21367010);广西矿冶与环境科学实验中心;广西危险废物处置产业化人才小高地资助项目

摘　　要：以茶皂素(tea saponin)为原料,以茶皂素降解率为指标,利用地衣芽孢杆菌降解茶皂素.分别探讨发酵时间、发酵温度、摇床转速、接种量对其降解的影响,再通过响应曲面设计实验对发酵条件进行优化.结果表明,影响降解率显著性次序为:发酵温度(X2)>接种量(X4)>发酵时间(X1)>摇床转速(X3),得到最佳的优化条件为:发酵时间10.9d,发酵温度28.89℃,摇床转速150r·min-1,接种量为9.11%.在此条件下发酵茶皂素的降解率达到了(63.83±0.28)%.Using tea saponin as raw material,with tea saponin degradation rate as the evaluation index,tea saponin was degraded by Bacillus licheniformis.Fermentation time,fermentation temperature,rotation speed and inoculum size on its degradation were explored,again through the response surface design experiments the fermentation conditions were optimized.The results showed that,the order of four kinds of factors significantly impacting the fermentation of Bacillus licheniformis as follows：fermentation temperature（X2）〉 inoculation size（X4）〉 fermentation time（X1）〉 rotation speed（X3）.The optimum fermentation degradation conditions were studied as：fermentation time 10.9d,fermentation temperature 28.89 ℃,rotating speed 150r·min-1 and inoculum size 9.11%,the degradation of tea saponin rate reached（63.83 ± 0.28）%.

关 键 词：茶皂素 地衣芽孢杆菌 液态发酵 响应曲面分析 

分 类 号：O156[理学—数学]