P21ras蛋白在喉鳞状细胞癌中的表达及临床病理意义  

Expression of P21ras protein in laryngeal squamous cell cancer and its clinicopathological significance

作　　者：刘彩双 吴铁男 赵立波[1] 安永宏[1] 项薇薇[1] 于海红[1] 

机构地区：[1]承德护理职业学院 [2]河北省承德市双桥区疾病预防控制中心,河北承德067000

出　　处：《临床和实验医学杂志》2016年第2期115-118,共4页Journal of Clinical and Experimental Medicine

基　　金：河北省高等学校科学技术研究项目(编号:Z2015011)

摘　　要：目的通过检测喉鳞状细胞癌组织、声带息肉、癌旁组织中P21ras蛋白的表达水平,探讨P21ras蛋白在喉鳞状细胞癌发生发展中作用及其机制,为喉鳞状细胞癌早期诊断供新的思路。方法收集60例喉鳞状细胞癌石蜡包埋组织,30例声带息肉及癌旁石蜡包埋组织,通过两步法免疫组织化学技术检测P21ras蛋白的表达水平,分析喉鳞状细胞中P21ras蛋白的表达及其与临床病理特征的关系。结果 P21ras蛋白在癌旁组织、声带息肉以及喉鳞状细胞癌中的阳性表达率分别为6.7%、36.7%和68.3%,三组间阳性率依次升高,三组间差异有统计学意义(P<0.05)。P21ras蛋白在中、高分化喉鳞状细胞癌中的阳性表达率为57.5%,在低分化喉鳞状细胞癌中的阳性表达率为90%,低分化组阳性表达率明显高于中、高分化组,两组间差异有统计学意义(P=0.011)。P21ras蛋白在Ⅰ期、Ⅱ期喉鳞状细胞癌中的阳性表达率为56.3%,在Ⅲ期、Ⅳ期喉鳞状细胞癌中的阳性表达率为83.1%,Ⅲ期、Ⅳ期组阳性表达率明显高于Ⅰ期、Ⅱ期组,两组间差异有统计学意义(P=0.021)。P21ras蛋白的表达与患者的性别、年龄、发病部位、有无淋巴结转移、有无远处转移无关(均P>0.05)。结论 P21ras蛋白在喉鳞状细胞癌中表达高于息肉及癌旁组织,并与肿瘤的分化及临床分期密切相关,表明P21ras蛋白可能在喉鳞状细胞癌的发生发展中发挥重要作用,检测P21ras蛋白对患者的早期诊断及预后有一定的指导意义。Objective To examine the expressions of P21 ras protein in laryngeal squamous cell carcinoma（ LSCC）,vocal cord polyp and carcinoma adjacent tissue,in order to explore the possible mechanism of LSCC development and provide new ideas for the early diagnosis of the disease. Methods In this study,60 cases of paraffin- embedded LSCC tissue specimens and 30 cases of carcinoma adjacent tissue and vocal cord polyp tissue were collected. The expression of P21 ras protein was detected by immunohistochemistry technique. The relationship between the expression of P21 ras protein and the clinicopathological characteristics of patients was studied. Results The positive rates of P21 ras protein in carcinoma adjacent tissue,vocal cord polyp tissue and LSCC tissue were 6. 7%,36. 7% and 68. 3% respectively. The positive rates were increased in succession,and there was significantly difference in P21 ras positive rate among the three tissue groups（ all P〈0. 05）. The higher expression of P21 ras protein was correlated with worse differentiation,and higher clinical stage（ all P〈0. 05）. But its expression was not correlated with sex,age,location,lymph node metastasis and distant metastasis（ P〉0. 05）. Conclusion The expression of P21 ras in LSCC is significantly higher than that in carcinoma adjacent tissue and vocal cord polyp and closely relates to tumor differentiation and clinical stage. P21 ras may play an important role in the development of LSCC. The detection of P21 ras has certain instructive significance for early diagnosis of LSCC and prognosis of patients.

关 键 词：喉鳞状细胞癌 P21RAS 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]