58例抗线粒体抗体M2亚型阳性患者检测结果分析  

Analysis of detection results of 58 cases of antimitochondrial antibody subtype M2 positive

作　　者：彭雪莲 史静[2] 邹麟[2] 董剑 王丹 

机构地区：[1]重庆市大足区人民医院检验科,400023 [2]重庆医科大学附属第一医院检验科,重庆400016

出　　处：《检验医学与临床》2016年第1期8-10,共3页Laboratory Medicine and Clinic

基　　金：国家临床重点专科建设项目[财社(2010)305]

摘　　要：目的研究抗线粒体抗体M2亚型(AMA-M2)在原发性胆汁性肝硬化(PBC)早期预防及诊治中的意义。方法选择58例AMA-M2阳性患者设为观察组,选择同期进行健康体检的30例健康者为对照组,再根据AMA-M2阳性的不同亚型,将观察组分为4个亚组,M2阳性且M4、M9阴性39例(A组),M2阳性且M4阳性4例(B组),M2阳性且M9阳性6例(C组),M2、M4、M9均阳性9例(D组)。应用全血动生化分析仪检测各组血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、γ-谷氨酰氨基转移酶(γ-GT)、总胆红素(TBIL)、直接胆红素(DBIL)的水平。结果观察组与对照组比较,ALP、γ-GT、TBIL、DBIL、ALT和AST水平,差异均有统计学意义(P<0.05)。AMA各亚型之间生化指标水平差异均有统计学意义(P<0.05)。结论 AMA-M2可作为PBC的筛查指标,联合检测M2、M4、M9亚型有助于PBC早期诊断和病情评估。Objective To study the significance of antimitochondrial antibody（AMA） subtype M2 in the early prevention,diagnosis and treatment of primary biliary cirrhosis （PBC）. Methods 58 eases of AMA-M2 positive were selected as the observation group and divided into the group A（M2 positive and M4,M 9 negative,39 cases） ,B（M4 positive,4 cases） ,C （M2 positive and M9 positive,6 cases） and D （M2,M4 and M9 positive,9 cases）. Contempora neous 30 healthy individuals of physical examination as the control group. Western blot was used to detect serum AMA-M2. The immunoblotting method was used to detect the M2,M4 and M9 in the patients with AMA-M2 posi tive. The automatic biochemical analyzer was applied to detect serum ALT, AST, ALP, 7-GT, TBIL and DBIL. Results The ALP,γ-GT,TBIL, DBIL, ALT and AST levels had the statistical difference between the observation group and the control group （P〈0.05）. The levels of biochemical indicators had statistical difference among different AMA subsets（P〈0.05）. Conclusion AMAM2 can serve as a screening index for PBC,and the combined detection of M2,M4 and M9 could conduce to the early diagnosis and condition assessment of PBC.

关 键 词：抗线粒体抗体 胆汁淤积 原发性胆汁性肝硬化 

分 类 号：R575.204[医药卫生—消化系统]