3个养殖大黄鱼群体线粒体DNA控制区遗传多样性分析  

Genetic diversity of Larimichthys crocea based on mitochondrial DNA control region sequences in three cultured populations

作　　者：沈静轶 薛良义[1] 沈伟良[1] 何萌萌[1] 

机构地区：[1]宁波大学海洋学院,宁波315211

出　　处：《生物学杂志》2015年第6期30-33,59,共5页Journal of Biology

基　　金：浙江省科技厅项目(2012C12907-8);宁波市科技局项目(2011B82018)资助

摘　　要：采用DNA测序技术,比较分析岱衢洋,普通闽-粤东族及“闽优一号”大黄鱼线粒体控制区的遗传多样性。98个样品扩增得到线粒体D-loop区序列长度785-799 bp,共检测出48种单倍型,其中在49尾岱衢洋大黄鱼呈现出17种单倍型,24尾普通闽-粤东族大黄鱼呈现出18种单倍型,25尾“闽优一号”大黄鱼呈现18种单倍型。3个养殖群体中共检测到167个有效的突变位点数目和39个插入/缺失位点;转换/颠换平均值为6.33。虽然单倍型多样性（Hd）平均达到0.8026,但核苷酸遗传多样性仍然很低。PM、DQ和MYOU三者的π值分别为0.01468,0.00266和0.01208,3个养殖群体的π值平均为0.00981。结果显示岱衢洋大黄鱼遗传多样性低于普通闽-粤东族,建议在人工选育过程中尽可能减少对群体遗传多样性的影响。Genetic diversity of mitochondrial DNA control regions of three cultured Larimichthys crocea populations, common Min-yue （ PM） , Dai-qu（ DQ） and ＆quot;Min-you1＆quot;（ MYOU） , were analyzed by DNA sequencing technology. The results showed that the D-loop sequence length of the 98 individuals ranged from 785 to 799 bp. The total number of haplotypes was 48, in which 18 were in both common Min-yue and ＆quot;Min-you1＆quot;, and 17 in Dai-qu. The effective numbers of polymorphic loci were 167, and insertion/deletion loci were 39 in three populations. The average of convert/transversion is 6. 33. Although the average number of haploid type diversity （Hd） was 0. 8026, the nucleotide diversity （π） remained low. The π volumes of three populations, PM, DQ and MYOU, were 0. 01468, 0. 00266 and 0. 01208, respectively, and the average number ofπwas 0. 00981. The results indicated that the genetic diversity of DQ population is far lower than that of PM . It was suggested that the effect of selection on genetic diversity should be taken care.

关 键 词：大黄鱼 线粒体DNA D-LOOP区 养殖群体 遗传多样性 

分 类 号：S917.4[农业科学—水产科学] S965.322