TLR2和TNF-α与输卵管炎的相关性研究  

Study on the relationship between expression of TLR2 & TNF-αand salpingitis

作　　者：陈晓[1,2] 王凤伟[3] 

机构地区：[1]金华市人民医院生殖中心,金华321000 [2]武义县中医院,金华321200 [3]浙江大学医学院附属妇产科医院中心实验室,杭州310012

出　　处：《生殖医学杂志》2016年第1期56-60,共5页Journal of Reproductive Medicine

基　　金：金华市科技计划项目(No.2014A33503)

摘　　要：目的建立输卵管炎模型,检测Toll样受体-2（TLR2）和肿瘤坏死因子-α（TNF-α）的表达,探讨其与输卵管炎的关系。方法 48只6~8周龄BALB/c雌性小鼠,随机平均分为空白组（不处理）和模型组（子宫底注入l×109/ml金黄色葡萄球菌悬液50μl）。分别在接种3d、7d、14d、28d处死模型组和对应时间的空白组小鼠,收集血液和输卵管。采用实时荧光定量PCR检测输卵管组织TLR2mRNA以及ELISA检测血TNF-α在模型组和相应空白组中的表达,并观察各组输卵管病理改变。结果空白组各时间点输卵管组织TLR2mRNA表达及血TNF-α浓度均无显著差异（P〉0.05）;模型组3d、7d、14d时TLR2mRNA表达逐渐降低,TNF-α浓度逐渐升高,但均无统计学差异（P〉0.05）,而28d时TLR2mRNA相对表达含量（0.822）显著低于3d时（1.296）（P〈0.05）;模型组各时间点血清TNF-α水平均显著高于相应空白组（P〈0.05）。结论TLR2可能通过一系列信号转导,诱导产生大量TNF-α,引起输卵管炎。Objective： To establish a salpingitis model for exploring the association between toll like receptor-2 （TLR2） ＆ tumor necrosis factor-α （TNF-α） and salpingitis. Methods： Forty-eight BALB/c female mice of 6-8 weeks were randomly divided to control group and model group. The mice in control group had no treatment,and the fundus of uterus of mice in model group was injected 50 μ 1× 109/ml suspension of staphylococcus aureus. The blood and fallopian tubes were collected after 3,7,14,28 days of treatment. The expressions of TLR2 mRNA in fallopian tubes and serum TNF-α levels were detected by using real-time fluorescence quantitative PCR and ELISA,respectively, and the pathological changes of fallopian tubes were observed in the model group and control group. Results： The expressions of TLR2 mRNA and serum TNF-α levels were not significantly different along with the time in the control group （P〉0.05）. The expressions of TLR2 mRNA were gradually decreased,while the concentrations of TNF-α were gradually increased after 3,7,14 days of treatment, but there were no significant differences （P〉0.05）. The TLR2 mRNA expression after 28 days of treatment was significantly lower than that after 3 days of treatment （P〈0.05）. The serum TNF-α levels in model group were all significantly higher than those in control group at corresponding time points （P〈0. 05）. Conclusions.. TLR2 might induce more TNF-α production through a series of signal pathways to cause salpingitis.

关 键 词：Toll样受体-2 肿瘤坏死因子-Α 输卵管炎 

分 类 号：R[医药卫生]