木犀草素对肝癌HepG2细胞线粒体自噬及Bcl-2表达的影响  

Effects of luteolin on mitophagy and Bcl-2 expression in HepG2 cells

作　　者：张怀念[1] 曹治家 陈红梅[1] 杨宝芸[2] 文颖[1] 柴栋[1] 张咏莉[1] 

机构地区：[1]广东药学院基础学院生物学教研室,广州510006 [2]广东药学院药科学院有机化学教研室,广州510006

出　　处：《广东医学》2015年第23期3586-3590,共5页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81102753)

摘　　要：目的探讨木犀草素对肝癌Hep G2细胞线粒体自噬及Bcl-2表达的影响。方法分别给予不同浓度的木犀草素处理肝癌Hep G2细胞。处理后,采用透射电子显微镜观察Hep G2细胞自噬体形成的超微结构,流式细胞仪检测Hep G2细胞线粒体膜电位变化及活性氧浓度,Lyso-Tracker Red荧光染色观察Hep G2细胞自噬情况,RT-PCR和Western blot技术检测Hep G2细胞Bcl-2 mRNA和蛋白的表达。结果木犀草素处理后的Hep G2细胞均出现线粒体损伤以及线粒体自噬现象,细胞线粒体膜电位显著降低(P<0.05),细胞内活性氧浓度显著升高(P<0.05),均出现酸性溶酶体增殖,并且呈浓度依赖性,细胞内Bcl-2 mRNA和蛋白表达量均显著降低(P<0.01)。结论木犀草素可诱导Hep G2细胞发生线粒体自噬,其机制与Bcl-2靶点表达下调相关。Objective To investigate the effects of luteolin on mitophagy and Bcl- 2 expression in Hep G2 cells.Methods Hep G2 cells were treated by different concentrations of luteolin. After luteolin treatment,autophagosome ultrastructure was observed with transmission electron microscopy,while the changes of mitochondrial membrane potential and concentration of reactive oxygen species in Hep G2 cells were measured by flow cytometry. Lyso- Tracker Red staining was used to observe autophagy in Hep G2 cells. Bcl- 2 mRNA and protein levels of expression in Hep G2 cells were detected by RT- PCR and Western blot,respectively. Results Hep G2 cells treated with luteolin showed mitochondrial damage and presence of mitophagosomes. A significant decrease in mitochondrial membrane potential and increase in levels of reactive oxygen species were observed（ P〈0. 05）. Acidic lysosomal proliferation in Hep G2 cells was also observed. The abundance of the above phenomena was dose- dependent. The mRNA and the protein expressions of Bcl- 2 were significant reduced（ P〈0. 01）. Conclusion Luteolin can induce mitophagy in Hep G2 cells and the mechanism may be associated with the down- regulated expression of Bcl- 2.

关 键 词：木犀草素 HEPG2细胞 线粒体自噬 BCL-2 

分 类 号：R735.7[医药卫生—肿瘤]