Raf-1和p-ERK在血管瘤裸鼠移植模型中的表达及意义  

Expressions of Raf-land ERK in xenografted human hemangioma

作　　者：徐艳朋[1] 俞松[1] 彭岗[1] 王健[1] 唐诗鹏[1] 罗宇[1] 

机构地区：[1]遵义医学院附属医院小儿矫形外科,563003

出　　处：《中华小儿外科杂志》2015年第12期936-939,共4页Chinese Journal of Pediatric Surgery

基　　金：国家自然科学基金（81160330,81460476）

摘　　要：目的动态观察Rab-1和ERK在裸鼠移植血管瘤中的表达，探讨其在血管瘤自然退化中的作用。方法动态观察移植瘤大小、形态变化，免疫组织化学检测Ra61、p-ERK1／2蛋白的分布及其表达情况，RT-PCR检测Raf-1mRNA、p-ERK1／2mRNA的表达情况。结果RaP1在增殖期移植血管瘤组织中的阳性表达率为100％，IOD值为89．39±9．67，Raf-1mRNA的相对定量为0．33±0．03；退化期移植血管瘤阳性表达率为55％，IOD值为60．73±9．66，Raf-1mRNA的相对定量为0．21±0．02，两者各项值比较，差异均有统计学意义（P〈0．01或P〈0．05）。p-ERK1／2在增殖期移植血管瘤组织中的阳性表达率为100％，IOD值为85．65±10．88，p-ERK1／2mRNA的相对定量为0．29±0．02；退化期移植血管瘤阳性表达率为63％，IOD值为49．78±11．20，p-ERK1／2mRNA的相对定量为0．20±0．02，两者各项值比较，差异均有统计学意义（P〈0．01或P〈0．05）。Raf-1蛋白与p-ERK1／2蛋白的表达量呈正相关关系（r=0．8550，P〈0．01），Raf-1mRNA与p-ERK1／2mRNA的相对定量呈正相关关系（r=0．8750，P〈0．01）。结论裸鼠移植的血管瘤中有Raf-1、p-ERK的表达；Ra51／ERK信号通路可能参与了血管瘤的发生发展过程。Objective To establish a reliable model of human hemangioma in nude mice for observing the expressions of Raf-1 and ERK in grafts so as to explore the roles of Raf-1/ERK signaling pathway in the occurrence, development and remission of pediatric hemangioma. Methods The expressions of Ra＆l and ERK in xenografted human hemangioma in proliferating and involuting phases were detected by hematoxylin-eosin staining, immunohistochemistry and RT-PCR （reverse transcription-polyrnerase chain reaction）. Results The expression rates of Raf-1 were 100% and 55% in proliferating and involuting phases respectively （P〈0. 01 ） ; IOD 89. 39 ± 9. 67 and 60. 73 ±9. 66 （P〈0. 05） ; Raf-lmRNA 0. 33 ±0. 03 and 0. 21 ± 0. 02 respectively （P〈0. 05）. The expression rates of ERK were 100% and 63% in proliferating and involuting phases （P〈0. 01 ）; IOD 85.65 ± 10. 88 and 49. 78 ± 11.20 （P〈0. 05） ; p-ERK1/2mRNA 0. 29± 0. 02 and 0. 20 ±0. 02 （P〈0. 05）. The protein expression levels of Raf-1 and p-ERK1/2 had a positive correlation （r = 0. 855 0, P〈0. 01）. And the mRNA relative quantities of Raf-1 and ERK had a positive correlation （r = 0. 8750, P〈 0. 01）. Conclusions Raf-1 and p-ERK protein and gene are expressed in xenografts of nude mice. And Raf-1/ERK signaling pathway may be involved in the occurrence of hemangioma.

关 键 词：血管瘤 裸鼠 动物模型 Raf-1/ERK信号通路 

分 类 号：R732.2[医药卫生—肿瘤] R-332[医药卫生—临床医学]