卵巢癌细胞株SKOV3中ERβ表达对细胞增殖凋亡影响的研究  

Expression of estrogen receptor beta activation on the proliferation and apoptosis in ovarian carcinoma cell SKOV3

作　　者：姚娇 葛书霞 丁朝霞 陈爱平 

机构地区：[1]山西省运城市中心医院,运城044000

出　　处：《现代妇产科进展》2015年第11期805-808,813,共5页Progress in Obstetrics and Gynecology

摘　　要：目的:研究卵巢癌细胞株SKOV3中ERβ表达对细胞增殖凋亡的影响。方法:MTT法检测卵巢癌细胞暴露于17β-E2不同浓度及不同时间的细胞增殖率。用特异性雌激素受体激动剂DPN联合17β-E2或单独使用DPN处理细胞,RT-PCR法检测ERβmRNA、ERK1/2 mRNA及caspase-3 mRNA表达,蛋白免疫印记法检测ERβ和p-ERK1/2蛋白表达。结果:10-6mol/L 17β-E2作用60min时,细胞的增殖活性最强(5.7±0.23),与对照组相比(3.5±0.45),差异有统计学意义(P<0.05)。ERβ在卵巢癌中低表达。随着DPN作用时间延长及浓度增加,ERβmRNA、caspase-3 mRNA表达逐渐增加(P<0.05),ERK2 mRNA表达逐渐降低,与对照组比较差异有统计学意义(P<0.05)。随着DPN作用时间延长及浓度的增加,ERβ蛋白表达逐渐增加,p-ERK1/2蛋白表达逐渐降低,与对照组比较差异有统计学意义(P<0.05)。结论:ERβ在卵巢癌中低表达,上调ERβ表达能抑制卵巢癌细胞的增殖,同时促进细胞的凋亡。Objective：To investigate the expression of estrogen receptor beta （ ER） ac-tivation on the proliferation and apoptosis in ovarian carcinoma cell. Methods：When the cells were exposed to 17β-E2 in different concentrations and for different time,the proliferation was assayed by MTT. After the cell was pretreated with DPN which was specific estrogen agonists and 17β-E2 or with DPN lonely,the expression of ERβ,caspase-3,ERK1,ERK2 mRNA were detected by RT-PCR,the protein expression of ERβ,p-ERK1/2 were detected by Western blot. Results：The cell proliferative rate was significantly higher in the cells（5. 7±0. 23） stimulated with 17β-E2 at 1＂10-6mol/L for 60 min than that in the control ones（3. 5±0. 45）（P〈0. 05）. The expression of ERβ decreased in the ovarian carcinoma. After the cells were treated with DPN,along with increasing of time and concentration, the expressions of ERβ and caspase-3 mRNA were increasingly higher than the control,while the expression of ERK1 mRNA,ERK2 mRNA were increasingly lower than that of control （ P〈0 . 05 ） . With increasing of time and con-centration,the protein expression of ERβ was increasingly higher than the control（P〈0. 05）. While the protein expression of p-ERK1/2 was increasingly lower than the control（P〈0. 05）. Conclusion：The expression of ERβ was decreased in ovarian carcinoma cells. When the ex-pression of ERβ was up-regulated, the proliferation was inhibited and the apoptosis was in-creased in ovarian carcinoma cell.

关 键 词：卵巢癌 雌激素受体Β 细胞增殖 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]