嗜酸乳酸杆菌对自主活动受限型应激小鼠肠道黏膜免疫状态的影响  

Influence of Lactobacillus acidophilus on Intestinal Mucosal Immunity in a Mouse Model of Stress Induced by Auto-Activity Restriction

作　　者：郝凤奇[1] 李景梅[1] 杨桂连[2] 

机构地区：[1]长春理工大学生命科学技术学院,吉林长春130022 [2]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《食品科学》2015年第23期247-252,共6页Food Science

基　　金：国家自然科学基金面上项目(31272552);吉林省教育厅科学技术研究项目

摘　　要：目的:探讨嗜酸乳酸杆菌(Lactobacillus acidophilus,LA)对自主活动受限型应激小鼠肠道黏膜免疫状态的影响。方法:以健康雌性BALB/c小鼠为受试动物,分为常规饲养组(NC组)、常规饲养条件灌胃LA组(NC+LA组)、应激组(S组)、应激条件灌胃LA组(S+LA组),小鼠的LA灌胃剂量为10~8CFU/d;应激组小鼠每天被限制自主活动3 h;15 d后取材,采用流式细胞术、酶联免疫吸附(enzyme-linked immunosorbem assay,ELISA)等方法,对肠系膜淋巴结(mesenteric lvmph nodes,MLN)中CD4^+T细胞、CD8^+T细胞和CDllc^+树突细胞(CDllc^+dendritic cell,CDllc^+DC)比例,结肠组织白细胞介素-10(intefleukin-10,IL-10)和白细胞介素-17(intefleukin-17,IL-17)水平,以及小肠总分泌型免疫球蛋白A(secreted immunoglobulin A,sIgA)水平进行检测。结果:常规饲养条件下,LA能极显著提高小鼠MLN中CD4^+T细胞比例(P<0.01),显著提高MLN中CDllc^+DC比例、小肠总sIgA水平和结肠组织IL-10水平(P<0.05),显著降低结肠组织IL-17水平(P<0.05),而对MLN中CD8^+T细胞比例无显著影响(P>0.05);应激条件能极显著降低小鼠MLN中CD4^+T细胞比例和CDllc^+DC比例(P<0.01),显著降低CD8^+T细胞比例(P<0.05),极显著降低小鼠结肠组织IL-10水平和小肠总sIgA水平(P<0.01),极显著提高结肠组织IL-17水平(P<0.01);应激条件下,LA能显著提高小鼠MLN中CD4^+T细胞比例和CDllc^+DC比例(P<0.05),显著提高小鼠结肠组织IL-10水平和小肠总sIRA水平(p<0.05),并显著降低结肠组织IL-17水平(p<0.05),而对小鼠MLN中CD8^+T细胞比例无显著影响(P>0.05)。结论:给予自主活动受限型应激小鼠LA干预,可提高小鼠MLN中CD4^+T细胞和CDllc^+DC比例,上调结肠IL-10和小肠总sIgA分泌水平,下调结肠IL-17分泌水平,调节应激小鼠的肠道黏膜免疫状态。Objective：To assess the influence of Lactobacillus acidophilus on intestinal mucosal immunity in a mouse model of stress induced by auto-activity restriction.Methods：Male health BALB/c mice were divided into 4 groups.In normal control group（NC）,the mice received balanced diet and water and were left undisturbed in their cages.In NC plus Lactobacillus acidophilus（NC ＋ LA） group,the mice were given a suspension of LA with 10~8 CFU/d per mouse through intragastric administration under normal control condition.In stress group（S）,the mice were subjected to stress induced by auto-activity restriction for 3 h per day.In stress plus LA（S＋LA） group,the mice were given a suspension of LA with 10~8 CFU/d per mouse through intragastric administration under stress condition.After 15 days,mice from each group were sacrificed.CD4~＋T,CD8~＋T and CD11c~＋ dendritic cells（CD11c~＋ DCs） in mesenteric lymph nodes（MLN）,interleukin-10（IL-10） and interleukin-17（IL-17） levels in colon tissue,total secretory immunoglobulin A（slgA） level in small intestine were analyzed by flow cytometry and enzyme-linked immunosorbent assay（ELISA）.Results：Under NC condition,the proportions of CD4~＋T cells and CD11c~＋DCs in MLN（CD4~＋T cell：P〈0.01,CD11c~＋DC：P〈0.05）,small intestinal sIgA level and IL-10 level in colon tissue（sIgA,IL-10：P〈0.05） were enhanced by LA and IL-17 level in colon tissue was decreased（P〈0.05）,but the proportion of CD8~＋T cells in MLN was not significantly varied（P〉0.05）.After auto-activity restriction,the proportions of CD4~＋T cells,CD8~＋T cells and CD11c~＋DCs,and the levels of IL-10 and small intestinal sIgA were decreased（CD4~＋T cell,CD11c~＋DC,IL-10,sIgA：P〈0.01,CD8~＋T cell：P〈0.05）.Interestingly,the proportion of CD4~＋T cells and CD11c~＋DCs,the levels of IL-10 and small intestinal sIgA were increased,and the level of IL-17 was decreased by LA under stress condition（P〈0.05）,but the proportion of CD8~＋T

关 键 词：嗜酸乳酸杆菌 自主活动受限 应激 肠道黏膜免疫 

分 类 号：Q939.91[生物学—微生物学]