蛋白酶基因的克隆与表达研究进展  

Progress in Cloning and Expression of Protease Genes

作　　者：王继莲[1,2] 李明源[1,2] 陈芸 

机构地区：[1]喀什大学叶尔羌绿洲生态与生物资源研究重点实验室,新疆喀什844006 [2]新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300

出　　处：《食品科学》2015年第23期377-381,共5页Food Science

基　　金：新疆生产建设兵团塔里木盆地资源保护利用重点实验室开放课题(BRYB1302);喀什大学校内青年专项课题((14)2526)

摘　　要：蛋白酶在各领域的应用愈加广泛,但其大规模工业生产受到诸多因素制约。利用基因工程技术对蛋白酶基因进行改良和克隆表达,开发高产量、高纯度、高活力的蛋白酶资源成为研究的热点。本文对蛋白酶的分类、结构与功能、菌种选育等进行了概述,同时分析了蛋白酶基因在原核和真核生物中的克隆与表达研究进展,并对未来蛋白酶基因工程的发展趋势进行了展望。Although proteases are widely applied in various fields, large-scale industrial application of proteases has been restricted by many factors. Genetic engineering technology has been used for the cloning and expression of protease genes to obtain the protease resources with high quality, high purity and high activity. The classification, structures and functions of proteases, and strain screening for protease production are reviewed in this article. Meanwhile, the cloning, and prokaryotic and eukaryotic expression of protease genes are discussed. Moreover, the future development in protease gene engineering technology is also prospected.

关 键 词：蛋白酶 基因工程技术 克隆表达 

分 类 号：Q786[生物学—分子生物学]