荷叶生物碱对THP-1源性巨噬细胞CD36及PPARγ表达的影响  

Effects of Nuciferine on CD36 and PPARγ Expressions of THP-1 Derived Macrophage

作　　者：沈白[1] 徐新[1] 张社兵[1] 陶军[2] 

机构地区：[1]韶关市粤北人民医院心内科,广东省韶关市512025 [2]中山大学附属第一医院心血管内科,广东省广州市510080

出　　处：《中国动脉硬化杂志》2015年第8期784-788,共5页Chinese Journal of Arteriosclerosis

基　　金：广东省科技计划项目(2013B021800091)

摘　　要：目的观察荷叶生物碱对THP-1源性巨噬细胞CD36和过氧化体增殖物激活型受体γ(PPARγ)表达的影响,探讨荷叶生物碱干预CD36表达及其信号传导途径。方法 THP-1单核细胞分化为巨噬细胞后,随机分为四组:空白对照组、ox-LDL组、荷叶生物碱组、环格列酮组,油红O染色观察THP-1巨噬细胞内脂质变化,RT-PCR和Western blot检测CD36和PPARγ的mRNA和蛋白表达水平。结果与空白对照组比较,经ox-LDL干预后,THP-1巨噬细胞内脂质蓄积增加,CD36和PPARγ的表达增加;与ox-LDL组比较,经荷叶生物碱干预后,THP-1巨噬细胞内脂质积蓄减少,CD36和PPARγ的表达减少;与荷叶生物碱组比较,经环格列酮干预后,THP-1巨噬细胞内脂质无明显变化,CD36和PPARγ的表达增加。结论荷叶生物碱通过下调CD36的表达抑制巨噬细胞泡沫化进展,荷叶生物碱可能是通过下调PPARγ来抑制CD36的表达。Aim To explore the effects of nuciferine on the expressions of CD36 and peroxisome proliferator-activated receptor γ（ PPARγ） in THP-1 derived macrophages,and the signal transduction pathway. Methods THP-1derived macrophages were randomly divided into groups of normal,oxidized low density lipoprotein（ ox-LDL）,nuciferine and ciglitazone. Macrophage cells were valued by oil red O staining. The expressions of CD36 and PPARγ were measured by RT-PCR and Western blot,respectively. Results Compared with normal group,lipid droplets were increased while mRNA and protein expressions of CD36 and PPARγ were upregulated in ox-LDL group. Compared with ox-LDL group,lipid droplets were decreased while mRNA and protein expressions of CD36 and PPARγ were downregulated in nuciferine group. Compared with nuciferine group,lipid droplets had no difference while mRNA and protein expressions of CD36 and PPARγ were upregulated in ciglitazone group. Conclusions Nuciferine downregulates CD36 expression in THP-1 derived macrophages,lessen the degree of foam formation. Nuciferine downregulates CD36 expression by downregulating PPARγ expression.

关 键 词：荷叶生物碱 氧化型低密度脂蛋白 环格列酮 CD36 过氧化体增殖物激活型受体Γ 

分 类 号：R363[医药卫生—病理学]