大豆基因组SSR和EST-SSR在黄芪中的通用性分析  

Transferability of Soybean Gemonic-SSR and EST-SSR Markers in Astragalus

作　　者：贺润丽[1] 樊杰[1] 平莉莉[1] 温旭东[1] 狄金雷 

机构地区：[1]山西中医学院,太原030024

出　　处：《分子植物育种》2015年第5期994-998,共5页Molecular Plant Breeding

基　　金：山西省国际科技合作项目(2012081045-1);山西中医学院博士科研启动基金项目(2014)共同资助

摘　　要：分子标记的种间通用性可降低其开发成本,提高利用效率,促进遗传研究较薄弱物种的分子遗传学研究。为开发黄芪SSR分子标记,本研究利用大豆的66对基因组SSR(G-SSR)和43对EST-SSR引物在黄芪基因组中进行通用性分析,并选出其中条带清晰易辨的23对引物对6种不同来源的黄芪进行遗传分析。结果表明:大豆G-SSR、EST-SSR引物在黄芪中的通用性比例分别为31.82%、76.74%,多态性引物分别占18.18%、53.49%,大豆EST-SSR在黄芪的通用性高于基因组SSR,且通用的大豆SSR标记可以用于不同来源黄芪的遗传多样性分析。本研究发掘的多态性SSR引物可以有效用于黄芪的分子遗传多样性研究。Transferability of molecular markers has the vital significance to reduce their development cost,improve their development efficiency and promote molecular genetics research of some species which genetic research is relatively weaker. For the development of SSR molecular markers in Astragalus, a popular Chinese medicinal plant, 66 pairs of genomic-SSR primers and 43 pairs of EST-SSRs primers designed from soybean were used to amplify the genomic DNA of Astragalus. Among 109 pairs of primers, 23 pairs of primers with distinct bands were selected to analyze genetic information of 6 Astragalus from different sources. The results showed that the transferability rate of soybean genomic-SSR and EST-SSR in Astragalus was 31.82% and 76.74%, respectively.The percentage of polymorphism of loci of effective genomic SSR primers was 18.18%, whereas EST-SSRs reached53.49% of polymorphism in Astragalus. The EST-SSRs of soybean had higher transferability to Astragalus than that of genomic-SSRs. The soybean SSR markers can be used for population genetic analysis and diversity analysis in Astragalus. The polymorphic SSR markers identified in this study are useful for further research of genetic diversity in Astragalus.

关 键 词：黄芪 大豆 SSR EST-SSR 标记通用性 

分 类 号：S567.239[农业科学—中草药栽培]