榕小蜂基因共表达研究(英文)  

Research on Co-expression Genes of a Fig Wasp

作　　者：崔东亚[1,2,3] 孙旭斌[1,2] 王佳佳[1,2] 张鹏[2] 孙宝发[4] 陈小伟[5] Robert W.Murphy 何顺民[2] 黄大卫[1,2] 

机构地区：[1]河北大学生命科学学院,保定071002 [2]中国科学院动物研究所系统与进化重点实验室,北京100101 [3]运城学院生命科学系,运城044000 [4]中国科学院基因组研究所,北京100101 [5]中国科学院生物物理研究所,北京100101 [6]Centre for Biodiversity and Conservation Biology, Royal Ontario Museum

出　　处：《生物化学与生物物理进展》2016年第1期83-89,共7页Progress In Biochemistry and Biophysics

基　　金：supported by grants from The National Basic Research Program of China(2011CB504605);The National Natural Science Foundation of China(31090253;31210103912)~~

摘　　要：榕小蜂的雌雄个体之间存在很大表型差异,以至于很难将雌雄个体彼此联系在一起.以对叶榕传粉榕小蜂作为材料,利用"加权基因共表达网络分析"软件(WGCNA),对榕小蜂的基因组和转录组进行分析,结果发现,5个基因共表达模块,分别用蓝色、蓝绿色、棕色、绿色和黄色标识,其中2个模块偏爱在雌蜂中表达,3个模块偏爱在蛹中表达.基因本体(GO)分析发现在蓝绿色和黄色表达模块中发现3个功能富集的基因集合.在蓝绿色基因表达模块中发现2个基因集合,分别与细胞周期和核苷酸结合活性有关;在黄色基因表达模块中发现1个基因结合,与细胞分化有关,尤其是与神经发育有关.Female fig wasps differ phenotypically from conspecific males to the extent that often they cannot be associated with oneanother. Weighted gene co-expression network analysis （WGCNA） of the genome and transcriptomes of one such fig wasp,Ceratosolensolmsi, generated five expression modules, which were flagged as blue, turquoise, brown, green and yellow. These involvedtwo female-biased expression modules and three pupa-biased expression modules, respectively. Gene ontotogies indicated threefunctional enrichment gene sets in modules turquoise and yellow. Two functional enrichment gene sets that participate in cell cycle orhave nucleotide binding activityclustered in turquoise module. The functionally enriched gene set in yellow module played roles in celldifferentiation, especially in neuron morphogenesis.

关 键 词：共表达 网络 功能富集 

分 类 号：Q7[生物学—分子生物学]