Wnt通路中部分基因在绒山羊胚胎皮肤毛囊中的表达  

Wnt Pathway Related Gene Expression in Skin Follicles of Cashmere Goat Embryo

作　　者：王乐乐[1] 张燕军[1] 杨坤[1] 韩文静[1] 郑亚光[1] 李晓燕[1] 王宏浩[1] 赵濛[1] 苏蕊[1] 王瑞军[1] 李金泉[1,2] 

机构地区：[1]内蒙古农业大学动物科学学院,呼和浩特010018 [2]内蒙古自治区动物遗传育种与繁殖重点实验室,呼和浩特010018

出　　处：《中国生物化学与分子生物学报》2016年第1期99-105,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家高技术研究发展计划(863计划;No.2013AA100506);国家农业部现代农业产业技术体系项目(No.CARS-40-05);内蒙古农业大学优秀青年科学基金(No.2014XYQ-1)资助~~

摘　　要：已知绒山羊毛囊的发育受Wnt等信号通路控制,但Wnt通路相关基因在绒山羊胚胎毛囊启动和生长发育过程中的表达及作用机制尚不清楚。本文采用RNA-Seq技术对45 d,55 d和65 d的绒山羊胚胎体侧皮肤进行了转录组测序,鉴定Wnt通路相关基因的表达。RNA-Seq技术结合blast搜索,将转录组有效测序数据与云南黑山羊参考基因组序列(http://goat.kiz.ac.cn/GGD/download.htm)比对,获得了已知的Wnt通路(pathway hsa04310)中的123个相关基因(86.0%)。进而采用实时荧光定量PCR技术检测,验证了差异表达的Sfrp4、Wnt3、Wnt10a(上调)和Apc2(下调)基因在绒山羊胚胎不同时期皮肤中的表达量,初步探索了绒山羊毛囊在胚胎期启动、发育过程中,Wnt通路部分基因的表达模式,为进一步研究Wnt通路部分基因在绒山羊胚胎毛囊启动、发育过程中的作用机制提供了有意义的线索。It has been reported that the hair follicle development of cashmere goats was regulated by several pathways including the Wnt signal. In the current study,RNA-Seq technology was used for the study of skin transcriptome. Samples were collected from the lateral body of 45,55 and 65 days cashmere goat embryo. The clean reads from RNA-Seq were aligned to the reference genome of Capra hircus breed Yunnan black goat（ http： / / ccb. jhu. edu / software / tophat / index. shtml）. The Wnt pathway（ hsa04310）,related 123 genes（ 86. 0%） of Wnt pathway have been identified. The expression of Sfrp4,Wnt3,Wnt10 a were up-regulated and Apc2 was down-regulated in different stages of embryonic skin development. Real-time quantitative PCR was used for validation. The expression profiling of the Wnt pathway from our results provided useful clues for the further study on the mechanism of the identified genes in follicles initiation and development in embryonic cashmere goat.

关 键 词：WNT通路 RNA-SEQ 毛囊 绒山羊胚胎 

分 类 号：Q75[生物学—分子生物学] S827[农业科学—畜牧学]