β-Conglycinin对不同发育时期鲤鱼消化酶活力的影响  

Effects of β-Conglycinin on activities of protease and amylase in juvenile and larval common carps

作　　者：邢秀苹 赖红娥[1] 赵晗[1] 杨欢欢 吴莉芳[1] 闫磊[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]厦门利洋水产科技有限公司,福建厦门361012

出　　处：《西北农林科技大学学报（自然科学版）》2016年第1期19-24,30,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：吉林省教育厅项目(2012043)

摘　　要：【目的】研究β-伴大豆球蛋白(β-Conglycinin)对鲤幼鱼、稚鱼蛋白酶和淀粉酶活力的影响。【方法】以初始体质量为(10.06±0.14)g/尾的鲤稚鱼和(110.23±0.23)g/尾的鲤幼鱼为研究对象,以鱼粉为动物蛋白源,面粉、糊精为糖源,混合油脂(m(鱼油)∶m(玉米油)=1∶1)为脂肪源,分别配制5种等氮(鲤幼鱼和稚鱼粗蛋白质量分数分别为36%和40%)、等能(鲤幼鱼和稚鱼总能分别是15.2和16.9 MJ/kg)的半精制饲料,其β-Conglycinin的添加量(质量分数)分别为0(CK),2.0%,4.0%,6.0%和8.0%,每组饲料设3个重复,在控温单循环养殖系统中进行为期8周的饲养试验,试验结束后,取鲤幼鱼、稚鱼的前、中、后肠道和肝胰脏,分别用福林-酚试剂法和淀粉酶试剂盒法,测定肠道和肝胰脏蛋白酶及淀粉酶的活力。【结果】鲤幼鱼肝胰脏蛋白酶活力各组之间差异不显著(P>0.05);β-Conglycinin添加量为6.0%和8.0%组的前肠、中肠蛋白酶活力显著低于对照组(P<0.05);而β-Conglycinin添加量为8.0%组后肠蛋白酶活力显著低于对照组(P<0.05)。在鲤稚鱼肝胰脏和后肠,β-Conglycinin添加量为8.0%组的蛋白酶活力显著低于对照组(P<0.05);前肠蛋白酶活力则以2.0%,4.0%,6.0%和8.0%添加组显著低于对照组(P<0.05);中肠蛋白酶活力为4.0%,6.0%和8.0%添加组显著低于对照组(P<0.05)。β-Conglycinin对鲤幼鱼和稚鱼肝胰脏、前肠、中肠及后肠淀粉酶活力均无显著影响(P>0.05)。【结论】鲤幼鱼配合饲料中β-Conglycinin的添加量不应超过6.0%;鲤稚鱼配合饲料中β-Conglycinin的添加量不应超过2.0%。[Objective] The research investigated the effects of β-Conglycinin on activities of protease and amylase in iuvenile and larval common carps. [Method] Larval and juvenile common carps with the ini- tial weights of （10. 06±0. 14） g/tail and （110. 23±0. 23） g/tail were used as experimental objects for eight-week feeding trial at controlled temperature in single recirculating system. Five diets with identical nitrogen （total crude protein contents for juvenile and larval were 36% and 40% ,respectively） and energy （total energies for juvenile and larval were 15.2 and 16.9 MJ/kg,respectively） as well as different β-Cong-lycinin contents （0（CK）,2. 0%,4. 0%,6. 0%,and 8.0%） were provided. Fis source, dextrin and flour were carbohydrate source, and mixed oil （m （corn oil） h meal was animal protein ： re（fish oil）=1 ： 1） was fat source. Each group had three repetitions. The activities of protease and amylase in foregut, midgut and hepatopancreas were detected using Folin-phenol method and Amylase kit. [Result] No significant effects of β-Conglycinin on activities of protease in hepatopancreas of juvenile common carps were observed （P） 0.05）. The activities of protease in foregut and midgut of juvenile common carps in 6.0% and 8.0% groups were significantly lower than that of the control group （P〈0.05） ,and that in hindgut of 8.0% group were significantly lower than that of the control group （P〈0.05）. The activities of protease in hepatopancreas and hindgut of larval common carps in 8.0% groups were significantly lower than that of the control group （P〈0.05） ,that of 2.0%,4.0%,6.0%,and 8.0% groups were significantly lower （P〈0.05） in foregut, and that of 4.0%,6.0% ,and 8.0% groups were significantly lower （P〈0.05） in midgut. In addition,no significant effects of β-Conglycinin on activities of amylase in hepatopancreas and tract of both juvenile and larval common carps were observed （P〉0.05）. [Conclusion] The amount of β-Conglycinin shoul

关 键 词：Β-伴大豆球蛋白 鲤 幼鱼 稚鱼 蛋白酶 淀粉酶 

分 类 号：S965.116.312[农业科学—水产养殖]