实时荧光定量检测心理疗法对宫颈癌化疗患者血清中C-myc和PTEN的影响  

Effect of psychotherapy on serum C-myc and PTEN in cervical cancer patients treated by chemotherapy using real-time fluorescence quantitative detection

作　　者：金秀琴[1] 

机构地区：[1]内蒙古民族大学附属医院,内蒙古通辽028007

出　　处：《中国妇幼保健》2016年第1期183-184,共2页Maternal and Child Health Care of China

基　　金：内蒙古自治区科技厅自然科学基金资助课题(2011MS1142)

摘　　要：目的观察心理疗法对宫颈癌化疗患者血清中C-myc和PTEN mRNA水平的影响。方法选择同期体检健康中年妇女20例作为对照组;58例来医院化疗的宫颈癌患者作为实验组,再随机分为常规护理组20例和心理护理组38例,两组均于化疗第1天及化疗第21天后清晨空腹采静脉血。采用实时荧光定量法检测C-myc和PTEN mRNA的表达水平。结果化疗前与对照组比较,常规护理组和心理护理组患者血清C-myc mRNA的表达水平均明显升高、PTEN mRNA的表达水平均明显降低,差异有统计学意义(P<0.01);常规护理组和心理护理组组间差异无统计学意义(P>0.05)。化疗后与化疗前比较,常规护理组和心理护理组患者血清C-myc mRNA的表达水平均明显降低、PTEN mRNA的表达水平均明显升高,差异有统计学意义(P<0.01);心理护理组下调C-myc mRNA水平及上调PTEN mRNA水平好于常规护理组,差异有统计学意义(P<0.05)。结论心理护理比常规护理能更好的下调C-myc mRNA水平、上调PTEN mRNA水平,这可能是能提高宫颈癌化疗后生存率的有效手段之一。Objective To observe the effect of psychotherapy on serum C-myc and PTEN mRNA levels in cervical cancer patients treated by chemotherapy. Methods Twenty healthy middle-aged women were selected as control group, 58 cervical cancer patients treated by chemotherapy were selected as experimental group and randomly divided into routine nursing group （20 cases） and psychological nursing group （38 cases） . Fasting venous blood specimens were abstracted on the first day and the 21st day after chcmotherapyl Real-time fluorescence quantitative method was used to detect the expression levels of C-myc and FTEN mRNA. Results Compared with control group, the expression levels of C-myc mRNA in routine nursing group and psychological nursing group before chemotherapy increased significantly, the expression levels of PTEN mRNA in routine nursing group and psychological nursing group before chemotherapy decreased significantly, there were statistically significant differences （P〈0. 01 ） ; there was no statistically significant difference between routine nursing group and psycho- logical nursing group （P〉0. 05） . Compared with before chemotherapy, the expression levels of C-myc mRNA in routine nursing group and psychological nursing group after chemotherapy decreased significantly, the expression levels of PTEN mRNA in routine nursing group and psychological nursing group after chemotherapy increased significantly, there were statistically significant differences （P〈0. 01 ） ; the decreasing amplitude of C-myc mRNA level and the increasing amplitude of PTEN mRNA level in psychological nursing group were statistically significantly better than those in routine nursing group （P〈0. 05 ） . Conclusion Compared with routine nursing, psychological nursing can down-regulate C-myc mRNA level and up-regulate PTEN mRNA level significantly, which may be one of effective methods to improve survival rate of cervical cancer patients after chemotherapy.

关 键 词：实时荧光定量 宫颈癌 心理疗法 原癌基因 抑癌基因 

分 类 号：R737.33[医药卫生—肿瘤]