抗氧化剂对过氧化氢处理的急性单核细胞白血病细胞增殖的影响  

Effect of antioxidants on the proliferation of acute monocytic leukemia cells treated with hydrogen peroxide

作　　者：路晨阳 白改改[1] 申秋菊[1] 蒙珊[1] 惠凌云[2] 苏丹[1] 张王刚[1] 周芙玲[1] 

机构地区：[1]西安交通大学第二附属医院血液内科,陕西西安710004 [2]西安交通大学第一附属医院检验科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2016年第1期39-44,53,共7页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81270597);2011年中央高校基本科研业务费专项资金资助项目(No.0602-08143041)~~

摘　　要：目的通过建立急性单核细胞白血病活性氧(ROS)细胞模型,观察抗氧化剂对其增殖的影响。方法选用人单核细胞白血病U937细胞株,用不同浓度过氧化氢(H_2O_2)处理,成功建立ROS细胞模型后给予不同浓度抗氧化剂超氧化物歧化酶(SOD)处理,检测细胞增殖、细胞内ROS含量、线粒体膜电位的变化。结果低浓度H2O2(50μmol/L)刺激U937细胞增殖;高浓度H_2O_2(500μmol/L)对U937细胞产生毒性作用。以50μmol/L H_2O_2处理U937细胞48h建立实验模型。正常U937细胞内存在少量ROS,少量凋亡细胞;50μmol/L H_2O_2处理后细胞内ROS增多,线粒体膜电位升高,凋亡率下降,细胞增殖旺盛,新合成的DNA增多;加入不同浓度抗氧化剂SOD,随着SOD浓度升高,ROS含量减少,线粒体膜电位下降,凋亡率增加,细胞增殖减慢,新合成的DNA减少,表现出剂量依赖性。结论抗氧化剂对急性单核细胞白血病细胞增殖有抑制作用,且表现剂量依赖性。Objective To observe the antioxidant effect on the proliferation of acute monocytic leukemia cells by establishing its reactive oxygen species （ROS） cell model. Methods U937 cell treated with different concentrations of hydrogen peroxide （H2Oz） was used to establish ROS cell model. The proliferative cells were treated with various concentrations of antioxidant superoxide dismutase （SOD）. We detected cell proliferation, intracellular content of ROS and mitochondrial membrane potential changes. Results （1） Low concentration of H2O2（50μmol/L） stimulated the proliferation of U937 cells while high concentration of H2O2 （500 μmol/L） produced a toxic effect on the cells. U937 cells were treated with 50μmol/L of H2O2 for 48 hours to establish the experimental model. （2） U937 ceils contained a small amount of ROS and apoptotic ceils; after 50 μmol/L H202 treatment, the content of intracellular ROS, the mitochondrial membrane potential, cell proliferation and the newly synthesized DNA were increased, but the apoptosis rate declined. After treatment with various concentrations of antioxidants superoxide dismutase （SOD）, the content of intracellular ROS, the mitochondrial membrane potential and the newly synthesized DNA decreased, and cell proliferation slowed down while the apoptosis rate was increased, which showed a dose-dependent manner. Conclusion Antioxidants can inhibit the proliferation of acute monocytic leukemia cells in a dose-dePendent manner.

关 键 词：急性单核细胞白血病 活性氧自由基(ROS) 抗氧化剂 细胞增殖 线粒体膜电位 超氧化物歧化酶(SOD) 

分 类 号：R733.7[医药卫生—肿瘤]