SALL4在肝癌中的表达及其对预后的影响  

Expression and prognostic significance of SALL4 in hepatocellular carcinoma

作　　者：王亚婷[1] 何彩霞[1] 卢振辉[2] 王琦[2] 杨少奇[3] 李洋 

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]宁夏医科大学总医院肝胆外科 [3]宁夏医科大学总医院消化内科,宁夏银川750004 [4]宁夏吴忠新区医院,宁夏银川751100

出　　处：《西安交通大学学报（医学版）》2016年第1期113-117,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：宁夏回族自治区对外科技合作项目~~

摘　　要：目的 检测人类婆罗双树样基因4（SALL4）在肝癌中的表达情况,探讨SALL4表达与原发性肝细胞癌（HCC）患者预后的相关性。方法 应用免疫组织化学法检测60例石蜡HCC标本及其中32例癌旁组织标本SALL4蛋白的表达,分析其与HCC患者临床病理学资料及预后的关系;采用荧光定量RT-PCR、Western blot法,分别检测23例新鲜HCC及癌旁肝组织中SALL4mRNA和蛋白的相对含量。结果 HCC组织中SALL4蛋白的表达率（81.67%,49/60）显著高于癌旁组织（6.25%,2/32）,差异有统计学意义（χ~2=48.047,P〈0.01）;SALL4蛋白在HCC中的表达与甲胎蛋白水平有关（P〈0.05）;SALL4高表达和低表达组患者的平均生存时间分别为（26.20±2.37、34.05±1.26）个月,SALL4高表达组患者的1、3年生存率（75.9%、55.2%）显著低于SALL4低表达组1、3年生存率（95.0%、85.0%,χ~2=5.044,P〈0.05）。肝癌及癌旁组织的SALL4mRNA的相对量分别为14.80±8.62、0.17±0.13,两组比较差异有统计学意义（t=8.14,P〈0.01）。Western blot检测结果显示,SALL4蛋白在HCC中的相对量为0.354±0.103。癌旁组织中几乎不表达。结论 SALL4表达与肝癌患者预后密切相关,可作为肝癌患者判断预后的预测指标。Objective To evaluate the expression of human spalt-like transcription factor 4 （SALL4） in hepatocellular carcinoma （HCC） and its relationship with the prognosis of primary HCC. Methods Immunohistochemical method was used to detect SALL4 protein expression in 60 cases of HCC paraffin specimens and 32 cases of corresponding paracarcinoma tissue paraffin specimens. We explored the relationship of SALL4 expression with the patients＇ clinicopathological data and the prognosis of HCC. Meanwhile, fluorescence quantitative reverse transcription polymerase chain reaction （FQ-RT-PCR） and Western blot were used to detect SALL4 expression in another 23 pairs of tumors and matched peritumoral samples of HCC with radical resection. Results The high expression rate of SALL4 protein （81.67%, 49/60） in HCC tissues was higher than that in paracarcinoma tissues （6.25%, 2/32）, with a significant difference （ x2 = 48. 047, P〈0.01）. The expression of SALL4 in HCC was correlated with AFP （P 〈 0. 05）. The average survival time for patients in high SALL4 expression group and low SALL4 expression group was 26. 20 ± 2. 37 and 34.05± 1. 26 months, respectively. Moreover, 1-year survival rate （75. 9%） and 3-year rate （55. 2%） in high SALL4 expression group were significantly lower than those in low expression group （95.0%, 85.0%, x2 =5.044, P〈0.05）. SALL4 mRNA in HCC and paracarcinoma tissues was 14.80±8.62 and 0.17 ± 0.13, respectively, with a significant difference （ t = 8.14, P〈0.01）. Western blot results showed the relative expression of SALL4 protein in liver cancer tissues was 0.354±0. 103, but SALL4 protein was hardly expressed in paracarCinoma tissues. Conclusion SALL4 expression is closely related to the postoperative prognosis of patients with HCC. Therefore, it can be used as a predictor for prognosis of liver cancer patients.

关 键 词：肝癌 SALL4基因 免疫组织化学 WESTERN BLOT 实时荧光定量PCR 

分 类 号：R735.7[医药卫生—肿瘤]