氧化苦参碱抗猪血清诱导的免疫性大鼠肝纤维化作用研究  

Effects of Immuno-Hepatic Fibrosis of Oxymatrine Anti-Porcine-Serum-Induced Rats

作　　者：赵宏伟 柏兆方[2] 张振芳[2] 崔鹤蓉[2] 张龙霏[3] 

机构地区：[1]江西中医药大学药学院,江西南昌330004 [2]中国人民解放军第三O二医院全军中医药研究所,北京100039 [3]山东中医药大学实验中心,山东济南250355

出　　处：《成都大学学报（自然科学版）》2015年第4期319-321,共3页Journal of Chengdu University（Natural Science Edition）

基　　金：国家自然科学基金(813300486)资助项目

摘　　要：目的:研究氧化苦参碱对猪血清诱导的大鼠免疫性肝纤维化的影响.方法:采用猪血清诱导的肝纤维化模型,肝脏病理学检测天狼星红染色观察纤维化程度,ELISA检测血清中TGF-beta1和IL-10的含量.结果:氧化苦参碱可明显降低实验大鼠肝脏胶原沉积,肝脏羟脯氨酸含量相对于模型组显著下降(P<0.05);与模型组相比,治疗组血清ALT和TGF-beta1的含量显著降低(P<0.05),IL-10含量显著升高(P<0.05).结论:苦参素可明显降低试验所用大鼠肝脏纤维化程度,对猪血清诱导的大鼠肝纤维化有良好的防护作用,对IL-10表达的影响或是其作用机制之一.The paper is going to investigate the effects of oxymatrine（ OMT） on immuno-hepatic fibrosis of rats induced by porcine-serum（ PS）. The porcine-serum-induced hepatic fibrosis model is adopted and hepatic pathology is used to detect the sirus red stain to observe the fibrosis degree. ELISA tests the content of TGF-beta1 and IL-10 in serum. The results show that OMT can decrease the collagen deposition in the liver of model rats,and the hydroxyproline（ HYP） in liver dwindles obviously（ P 0. 05） compared with the model set. Then,compared with the model set,the content of serum ALT and TGF-beta1 of the treatment group decreases evidently（ P 0. 05） while the content of IL-10 increases obviously（ P 0. 05）. Finally,a conclusion is drawn that the OMT can decrease the hepatic fibrosis degree of the model rats,and can also well protect the porcine-serum-induced hepatic fibrosis rats. The influence on IL-10 expression might be one of OMT＇s functions.

关 键 词：氧化苦参碱 肝纤维化 猪血清 IL-10 TGF-beta1 

分 类 号：R575.2[医药卫生—消化系统] R285.5[医药卫生—内科学]