基于支持向量回归机的定经汤调节卵巢早衰作用机制研究  

Mechanism Research of Ding Jing Decoction on Regulating the Premature Ovarian Failure Based on the Support Vector Regression

作　　者：王秀凤[1] 张磊[1] 伍庆华[2] 闵建新[2] 马娜[1] 罗来成[1] 

机构地区：[1]广东药学院基础学院,广州510006 [2]江西中医药大学基础医学院,南昌330006

出　　处：《中国中医基础医学杂志》2015年第12期1516-1518,1565,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81073073)-基于人工神经网络建模的补肾疏肝药对菟丝子-柴胡调整卵巢早衰作用机制研究;国家自然科学基金资助项目(81403153)-基于支持向量回归机的右归丸调节肾阳虚的作用机制研究

摘　　要：目的:探讨定经汤调节卵巢早衰的作用机制。方法:SD大鼠按随机数字表法分为正常组、模型组、定经汤干预组,分别检测其与卵巢早衰相关的生物分子,如β-EP、IL-1、NOS、NO、Gn RH、CRH、FSH、LH、E2、P、ACTH和CORT;建立基于支持向量回归机的生物分子网络模型,探究定经汤干预下各生物分子之间相互调控关系。结果:卵巢早衰大鼠灌服定经汤后,垂体-卵巢轴及垂体-肾上腺轴靶腺层生物分子指标值上升,垂体-卵巢轴及肾上腺轴的变化反馈到下丘脑,使得IL-1、NOS和NO指标值上升;通过IL-1的枢纽作用,使得β-EP、Gn RH和CRH上升;最后下丘脑生物分子的上升又使得垂体-卵巢轴及肾上腺轴的靶腺层生物分子进一步上升,垂体-卵巢轴垂体层生物分子稍有下降。结论:定经汤干预下卵巢早衰相关生物分子网络趋于平衡,定经汤调节卵巢早衰过程中起到关键作用的生物分子为IL-1。Objective： Exploring the mechanisms of the premature ovarian failure（ POF） regulation by Dingjing Decoction. Methods： 90 SD rats were randomly divided into three groups： normal groups,model groups and Dingjing Decoction groups; Biomolecules which associated with premature ovarian failure： β-EP,IL-1,NOS,NO,Gn RH,CRH,FSH,LH,E2,P,ACTH and CORT were detected. Regulation relations of biomolecules associated with POF regulation by Dingjing Decoction were explored based on support vector regression machine. Results： The values of biomolecules in pituitary-ovarian axis and target gland layer of pituitary-adrenal axis increased significantly in curing with Dingjing Decoction; then these changes feed back to the hypothalamus,leading the increase of the values of IL-1,NOS and NO in hypothalamus; then the values of β-EP、Gn RH and CRH increased in hypothalamus through the pivotal function of IL-1;The values of biomolecules in target gland layer of pituitary-adrenal axis and pituitary-ovarian axis increased significantly,the values of biomolecules in the pituitary layer of hypothalamic-pituitary-ovarian axis decreased slightly,when the values of biomolecules in hypothalamus increased. Conclusions： The regulation relations of biomolecules network associated with to be balance. IL-1 play a pivotal role in the biomolecules network associated with POF regulation by Dingjing Decoction.

关 键 词：定经汤 卵巢早衰 作用机制 支持向量回归机 

分 类 号：R289[医药卫生—方剂学]