高效液相色谱-串联质谱同时测定草莓、杨梅中20种植物生长调节剂残留  

Simultaneous Determination of 20 Plant Growth Regulator Residues in Strawberry and Chinese Bayberry(Myrica rubra) by High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：虞淼 吴淑春[2] 

机构地区：[1]浙江省农药检定管理所,浙江杭州310020 [2]浙江医学高等专科学校,浙江杭州310053

出　　处：《食品科学》2015年第24期246-252,共7页Food Science

基　　金：浙江省科技厅分析测试科技计划项目(2013C37069);浙江省教育厅高等学校访问工程师校企合作项目(FW2013009)

摘　　要：建立一种高效液相色谱-串联质谱同时测定草莓和杨梅中20种植物生长调节剂残留的分析方法.样品经含1％乙酸的乙腈溶液提取后,以Phenomenex XB-C18（100 mm×2.1mm,2.6 μm）为分析色谱柱,甲醇和5 mmol/L乙酸铵-0.1％乙酸缓冲溶液为流动相进行梯度洗脱,基质匹配标准溶液外标法定量.结果表明,20种植物调节剂在各自的质量浓度范围内线性关系良好（r≥0.990 0）,草莓和杨梅中3个添加量的回收率为70.0％～114.8％,相对标准偏差（n=5）为1.25％～11.1％,检出限在0.14～2.9 μg/kg之间.该方法操作简单、提取效果好,具有良好的灵敏度、回收率和重复性.The present paper describes the development and validation of a method for the simultaneous determination of 20 plant growth regulator residues in strawberry and Chinese bayberry （Myrica rubra） by using high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）. The plant growth regulator residues in 15 g of samples were extracted with acetonitrile （containing 1% acetic acid） and then analyzed using HPLC-MS/MS. The chromatographic analysis was carried out on a Phenomenex XB-C18 （100 mm× 2.1 mm, 2.6 tim） column with methanol and 5 mmol/L ammonium acetate solution containing 0.1% acetic acid as the mobile phases with a gradient program, and the analytes were quantified by matrix-matched external standard method. The recoveries of 20 plant growth regulators in strawberry and Chinese bayberry at three spiked levels were 70.0%-114.8%, with relative standard deviation （RSD） between 1.25% and 11.1%. The limits of detection of the method ranged from 0.14 to 2.9 μg/kg. The method is simple, rapid, sensitive, accurate and repeatable. These figures of merit meet the requirements for the detection of 20 plant growth regulator residues in strawberry and Chinese bayberry.

关 键 词：高效液相色谱-串联质谱 植物生长调节剂 草莓 杨梅 残留 

分 类 号：O657.63[理学—分析化学]