阿尔茨海默病患者外周血白细胞介素23和白细胞介素17测定及意义  

The determination and significance of interleukin 23 and interleukin 17 in peripheral blood of patients with Alzheimer's disease

作　　者：钟城垚 周定群 刘建英[1] 

机构地区：[1]遵义医学院第三附属医院老年病科,贵州遵义563000

出　　处：《中风与神经疾病杂志》2015年第12期1102-1105,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨阿尔茨海默病(Alzheimer’s disease,AD)患者外周血中炎症因子白细胞介素23(Interleukin-23,IL-23)和IL-17水平及其与认知功能损害的相关性。方法纳入AD患者102例(研究组),以及健康老年志愿者100例(对照组)。搜集受试者的一般资料和疾病信息。采用简易精神状态量表(MMSE)和临床痴呆评定量表(CDR)对受试者认知功能和日常行为能力进行评估。应用实时荧光定量逆转录-聚合酶链式反应(RTPCR)检测各组入选者外周血淋巴细胞中IL-23和IL-17的mRNA表达,应用酶联免疫吸附试验(ELISA)检测入选者血清IL-23和IL-17蛋白的表达。比较两组入选者血清炎症因子mRNA及蛋白水平的差异,分析外周血IL-23和IL-17表达的相关性。以全部入选者为研究对象,分析两组IL-17和IL-23表达差异与MMSE评分的相关性。以AD组为研究对象,分析患者IL-17和IL-23表达水平与CDR评分的相关性。结果 AD组外周血淋巴细胞中IL-23和IL-17的mRNA表达水平及血清IL-23和IL-17蛋白的表达水平均显著高于对照组,差异均有统计学意义(P<0.05),且两者具有显著正相关性(P<0.01)。整体人群中,IL-23和IL-17水平均与MMSE评分呈负相关(P<0.05)。AD患者血IL-23和IL-17水平与CDR评分均无相关性。结论外周血IL-23和IL-17水平与整体人群的认知功能相关,但与AD患者的痴呆严重度无明显相关性。Objective To investigate the relationship between the levels of interleukin 23 and interleukin 17 in peripheral blood of patients with Alzheimer＇ s disease and cognitive impairment. Methods 102 AD patients （ study group） and 100 healthy aged volunteers （control group） were recruited in the present study. The general information and disease information of the subjects were collected. Mini-Mental State Examination （MMSE） and Clinical Dementia Rating Scale （CDR） were used to assess the cognitive function and activities of day living of the subjects. Real-time fluorescent quantitative reverse transcription polymerase chain reaction （RT-PCR） was used to detect the expression of IL-23 and IL-17 mRNA in＇peripheral blood lymphocytes of the patients, and the expression of IL-23 and IL-17 proteins was detected by enzymelinked immunosorbent assay （ELISA）. The differences of peripheral blood inflammatory factors mRNA and protein levels in the two groups were compared, and the correlation between the expression of 1L-23 and IL-17 was analyzed. Partial correlation analysis was performed to study the correlation between inflammatory cytokine levels of all subjects and MMSE scores in the whole sample, and the correlation between the serum levels of inflammatory cytokines and CDR scores was examined in the AD cohort. Results The expression levels of IL-23 and IL-17 mRNA and proteins in peripheral blood of AD group were significantly higher than that of control group （ P 〈 0.05 ） , both had a significant positive correlation （ P 〈 0.01 ）. The levels of IL-23 and IL-17 were negatively correlated with MMSE score （ P 〈 0.05 ） in the whole sample. No significant correlation was observed between serum inflammatory factors and CDR scores in AD patients. Conclusions The peripheral blood levels of IL-23 and IL-17 may be correlated with the cognitive function but not with the severity of dementia in AD patients.

关 键 词：阿尔茨海默病 IL-23 IL-17 

分 类 号：R749.1[医药卫生—神经病学与精神病学]