一株海洋锰氧化真菌的分离、鉴定及其对Mn(Ⅱ)的氧化特性  

Identification of a manganese-oxidizing fungus isolated from marine sediment and its Mn(Ⅱ) oxidation characteristics

作　　者：王文秀[1] 马靖宇[1] 朱祎捷 崔鸿鹏[1] 魏士平[1] 

机构地区：[1]中国地质大学(北京)海洋学院,北京100083 [2]Faculty of Science, University of Waterloo, Waterloo N2L 3GI, Canada

出　　处：《应用与环境生物学报》2015年第6期1032-1036,共5页Chinese Journal of Applied and Environmental Biology

基　　金：大学生创新创业训练计划教学实验室开放项目(国家级)(201411415067)资助~~

摘　　要：从北戴河海洋沉积物中共分离出120株真菌,经筛选得到一株锰氧化能力较强的菌株PSA-107h.经ITS测序并比较,该菌株与Ascomycete中的菌株有较高的同源性.随着Mn^(2+)浓度的增高,其对锰的氧化能力逐渐增强,但在高浓度Mn^(2+)时,其生长会受到一定的抑制.另外,发现PSA-107h在pH 5.0-9.0范围内都能对锰进行氧化,且在pH 6.0时对锰氧化的程度较高.通过显微镜观察,发现真菌对锰的氧化主要在胞间进行,且随着菌丝生长时期的延长,锰氧化程度逐渐升高.PSA-107h在7 d内对液体中1 mmol/L的Mn^(2+)的去除效率可达88.6%.本研究为微生物除锰提供了良好的菌种资源.bstract Microbial organisms play an important role in oxidation of Mn^2＋ in the natural environment. In this research we isolated a total of 120 fungal strains from the sediment collected from Beidaihe and identified out of them a manganeseoxidizing strain PSA-107 h. ITS sequence analysis showed that it was closely affiliated to Ascomycota sp. Experiment of its growth on media containing different manganese concentrations showed that the strain PSA-107 h had greater manganese oxidation in higher manganese concentration, but very high manganese concentration would inhibit its growth. Manganese oxidation by PSA-107 h could occur in a wide range of pH from 5.0 to 9.0, with the optimal pH of 6.0. Microscopy analysis revealed that the manganese oxidation was intercellular and becoming stronger with older mycelium. In liquid medium, the manganese removal of PSA-107 h could reach 88.6%. This strain has great potential in industrial scale manganese removal from contaminated water.

关 键 词：锰氧化真菌 ASCOMYCETE 锰氧化 锰去除 锰氧化物 

分 类 号：X172[环境科学与工程—环境科学]