单个B细胞抗体制备技术及其在肝脏疾病中的应用  

Application of antibody preparation technology based on single B lymphocytes in liver diseases

作　　者：吕信萍 吴静[1] 陈京涛[1] 

机构地区：[1]吉林大学白求恩第一医院转化医学研究院,长春130021

出　　处：《临床肝胆病杂志》2015年第12期2104-2109,共6页Journal of Clinical Hepatology

基　　金：科技部"十二五"艾滋病和病毒性肝炎等重大传染病防治项目子课题(2013ZX10004609-004);吉林省科技厅项目(20140414014GH;2014N147)

摘　　要：单克隆抗体是具有高度特异性的抗体,目前已广泛应用于多种疾病,尤其是在各种病毒引发的肝损伤的治疗和研究中,显示出了不可替代的作用。近年来基于单个B淋巴细胞直接制备的人源性单克隆抗体具有抗原特异性高、免疫原性低及安全高效等优点,越来越受到瞩目。随着单克隆抗体技术和PCR技术的不断改进,单个B淋巴细胞抗体制备技术也有了很大发展。尽管由单个B淋巴细胞产生作用于肝脏的单克隆抗体的相关报道并不多见,但其在肝脏疾病的治疗和研究中展现出了广阔的应用前景。简述了单个B淋巴细胞抗体制备技术的研究进展及单个B淋巴细胞制备的抗体在肝脏疾病中的应用。Monoclonal antibodies（ m Abs） have a high specificity and have been widely used in various diseases,especially in the treatment and study of liver injury induced by viruses. In recent years,human m Abs directly prepared from single B lymphocytes have been getting more and more attention due to high antigenic specificity,low immunogenicity,and good safety and efficiency. With the continuous improvement in monoclonal antibody technology and polymerase chain reaction technology,the antibody preparation technology based on single B lymphocytes has been developed greatly. Although there are rare reports about the m Abs which are derived from single B lymphocytes and applied in liver diseases,the application of such m Abs is promising in the treatment and study of liver diseases. This article reviews the research advances in the development of antibody preparation technology based on single B lymphocytes and the application of the prepared antibodies in liver diseases.

关 键 词：肝疾病 抗体 单克隆 单个B淋巴细胞 综述 

分 类 号：R575[医药卫生—消化系统]