平喘宁对哮喘大鼠肺组织TGF-β1、CyclinD1及p-ERK1/2mRNA表达的影响  

Influence of Pingchuanning on expression of TGF-β1,CyclinDl and p-ERK1/2 mRNA in lung tissues of asthma rats

作　　者：方向明[1] 王智星[1] 邬锡琴[2] 

机构地区：[1]安徽中医药大学,合肥230038 [2]合肥工业大学,合肥230009

出　　处：《中华中医药杂志》2016年第1期296-299,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81173187)~~

摘　　要：目的:观察平喘宁对寒性哮喘大鼠肺组织中转化生长因子-β1(TGF-β1)、细胞周期蛋白D1(Cyclin D1)和磷酸化细胞外信号调节激酶1、2(p-ERK1/2)m RNA表达的影响。方法:雄性SD大鼠105只,随机分为正常组、模型组、桂龙咳喘宁组、地塞米松组、平喘宁高剂量组、平喘宁中剂量组、平喘宁低剂量组,每组15只。以卵蛋白致敏及寒冷刺激复制大鼠哮喘模型,造模21d后,正常组、模型组每天给予蒸馏水灌服,其它各组给予相应药物灌服,4周后解剖大鼠并取出肺组织。采用免疫组化法测定肺组织中TGF-β1、Cyclin D1的含量,采用RT-PCR检测平喘宁治疗哮喘前后p-ERK1/2 m RNA表达的变化。结果:和正常组比较,模型组发生明显炎性细胞侵润,气道平滑肌增厚;肺组织TGF-β1、Cyclin D1的表达水平上升(P<0.05,P<0.01);p-ERK1/2 m RNA表达水平提高(P<0.05,P<0.01)。和模型组比较,各治疗组肺组织病理改变有不同程度的好转;TGF-β1、Cyclin D1表达下降,p-ERK1/2 m RNA表达水平降低(P<0.05,P<0.01)。结论:平喘宁可通过抑制P-ERK1/2 m RNA表达,调节ERK信号转导通路,抑制TGF-β1、Cyclin D1表达,抑制气道重塑而治疗哮喘。Objective：To study the influence of Pingchuanning on the expression of TGF-β1,CyclinD1 and p-ERK 1/2mRNA in lung tissue of asthma rats.Methods：105 male SD rats were randomly divided into normal group,model group,Guilong Kechuanning group,dexamethasone group,high,middle and low dose of Pingchuanning groups,and with 15 rats in each group.The stimulations as ovalbumin sensitization and cold were used to establish the asthma rat model.After 21 days,rats in normal group and model group received gavage administration with distilled water,and rats in the other groups received gavage administration with corresponding drugs for 4 weeks.The contents of TGF-β1 and CyclinDI in lung were tested by suing immunohistochemistry method,and the expression of p-ERK1/2 mRNA was determined by using RT-PCR.Results：Compared with normal group,there was obvious inflammatory cell invasion in lung tissue of rats in model group,the airway smooth muscle was thickened,and the contents of TGF-β1,CyclinD1 and expression of p-ERK1/2 mRNA in lung tissue of rats in model group was increased（P〈0.05,P〈0.01）.Compared with model group,the pathological changes of rats in the treatment groups were all improved to some extent,and the contents of TGF-β1 and CyclinD1 and the expression of p-ERK1/2 mRNA in lung tissue of rats in treatment groups were all decreased（P〈0.05,P〈0.01）.Conclusion：Pingchuanning could treat asthma through inhibiting the expression of p-ERK1/2 mRNA,TGF-β1 and CyclinD1,regulating ERK signal transduction pathway,and inhibiting airway remodeling.

关 键 词：哮喘 平喘宁 TGF-Β1 CYCLIN D1 P-ERK1/2 m RNA 

分 类 号：R285.5[医药卫生—中药学]