基于鱼类膳食的重金属混合物联合毒性研究  

Study: Joint Toxicity of Heavy Metal Mixtures Related to Proportions of Fish Consumption

作　　者：林夏露 谷元亮 周琪[1] 毛国传[1] 马东杰[1] 赵进顺[1] 邹宝波[1] 

机构地区：[1]宁波大学医学院,浙江宁波315211

出　　处：《宁波大学学报（理工版）》2016年第1期22-27,共6页Journal of Ningbo University：Natural Science and Engineering Edition

基　　金：国家自然科学基金(81273111);宁波市科技计划项目(2012C5019);浙江省公益性技术应用研究计划项目(2015C33148)

摘　　要：为探讨Pb、Cd、Hg、Cu、Zn、Mn、Cr、Ni 8种重金属的联合毒性,按宁波地区居民鱼类膳食重金属的暴露比例配制各种混合物,比较单种重金属以及七元及八元重金属混合物对JB6小鼠上皮细胞的细胞增殖活力影响(MTT法).结果显示,鱼类膳食各重金属暴露比例为Zn>Cu>Mn>Cr>Ni>Cd>Pb>Hg;在JB6细胞系,单种重金属对细胞的毒性大小为Hg>Cd>Cr>Zn>Pb>Cu>Mn>Ni;多元重金属混合物对细胞的毒性大小为m8-Mn组>m8-Ni组>m8-Pb组>m8-Cu组>m8-Zn组>m8-Hg组>m8-Cd组>m8-Cr组>m8组.毒性评价法、相加作用模型及联合作用模型比较均表明,各重金属混合物内的重金属之间的主要联合作用为协同作用或相加作用,而Zn、Mn与其他重金属之间存在较强拮抗作用.To study the joint toxicity of lead, cadmium, mercury, copper, zinc, manganese, chromium and nickel, the single heavy metal and multicomponent mixtures are exposed to normal mice epithelial cells （JB6）, and the toxicity is measured by MTT tests. The ratios of heavy metal in mixtures are set according to the proportions on fish consumption in Ningbo area. The results show that the heavy metal proportions on fish consumption are in following descending order as Zn〉Cu〉Mn〉Cr〉Ni〉Cd〉Pb〉Hg. In JB6 cells, the single toxicities are found to be in order of Hg〉Cd〉Cr〉Zn〉Pb〉Cu〉Mn〉Ni, and the order for joint toxicities of different heavy metal mixtures are mS-Mn〉mS-Ni〉m8-Pb〉mS-Cu〉mS-Zn〉mS-Hg〉mS-Cd〉m8-Cr〉mS. The analysis of toxic units, additive model and combination model indicate that there are mainly synergistic or additive effects on the heavy metal mixtures. Zinc and manganese could be used to resist the toxicities of other metals.

关 键 词：鱼类 膳食 重金属 混合物 联合毒性 

分 类 号：R114[医药卫生—卫生毒理学]