IL-13对与成纤维细胞共培养的乳腺癌细胞表达Bcl-2和TIGAR的影响  

The effects of IL-13 on the expression of Bcl-2 and TIGAR in breast cancer cells co-cultured with fibroblasts

作　　者：李文林[1] 吴红[1] 孟闯[1] 陈少芬[2] 石小玉[2] 

机构地区：[1]南昌大学江西省医学生物高技术重点实验室,330006 [2]南昌大学组织学与胚胎学教研室,330006

出　　处：《免疫学杂志》2016年第2期93-97,共5页Immunological Journal

基　　金：国家自然科学基金(91229118)

摘　　要：目的探索白细胞介素-13(interleukin-13,IL-13)对乳腺癌作用的分子机制。方法采用Transwell小室方法共培养人乳腺癌细胞与人成纤维细胞;RT-q PCR和Western blot检测IL-13对乳腺癌细胞抗凋亡因子B-cell lymphoma-2(Bcl-2)和TP53-induced glycolysis apoptosis regulator(TIGAR)表达的影响;Annexin V和CCK-8实验检测IL-13对乳腺癌细胞凋亡和增殖的影响。结果 IL-13上调了与人成纤维细胞CCC-ESF-1(ESF)共培养的人乳腺癌细胞BT-474的抗凋亡因子Bcl-2和TIGARm RNA和蛋白的表达。与其他各组比较,BT-474+ESF+IL-13组BT-474细胞的Bcl-2和TIGAR表达显著上调(P<0.05);加入IL-13可抑制BT-474细胞的凋亡,与BT-474+ESF共培养组比较,BT-474+ESF+IL-13共培养组的早期调亡的BT-474细胞下降了10.85倍;IL-13促进了BT-474细胞增殖,BT-474+ESF+IL-13组与各组比较,培养48 h、72 h、96 h和120 h后,BT-474细胞的增殖显著高于其它各组(P<0.05)。结论 IL-13可上调与人成纤维细胞共培养的人乳腺癌细胞BT-474抗凋亡因子Bcl-2和TIGAR的表达;IL-13对乳腺癌作用的分子机制涉及Bcl-2和TIGAR。To explore the molecular mechanism of IL-13 in the breast cancer, human breast cancer cells and human fibroblasts were co-cultured by Transwell inserts method, and the effects of IL-13 on the expression of anti-apoptosis factors B-cell lymphoma-2 （Bcl-2） and TP53-induced glycolysis apoptosis regulator （TIGAR） in breast cancer cells were detected by RT-qPCR and Western blotting methods. Then, the effects of IL-13 on the apoptosis and proliferation of breast cancer cells were examined by Annexin V and CCK-8 methods. Data showed that compared with other groups, Bcl-2 and TIGAR expression of BT-474 ceils were significantly up-regulated in BT-474＋ESF＋IL-13 group （P〈0.05）, and addition of IL-13 inhibited the apoptosis of BT-474 cells; compared with BT-474 ＋ ESF co-culture group, the early apoptotic BT-474 cells of BT-474 ＋ ESF ＋ IL-13 co-culture group decreased by 10.85-fold; BT-474 cell proliferation was significantly greater in BT-g7g ＋ ESF ＋ IL- 13 co-culture group than that of other groups at 48, 72, 96, and 120 h （P〈O.05）. These results demonstrated that IL-13 can up-regulate the expression of anti-apoptotic factors Bcl-2 and TIGAR of human breast cancer cells BT-474 co-cultured with human fibroblasts ESF, and the molecular mechanism of the effect of IL-13 on breast cancer involves Bcl-2 and TIGAR.

关 键 词：白细胞介素-13 BCL-2 TIGAR 乳腺癌 成纤维细胞 

分 类 号：R737.9[医药卫生—肿瘤]