基于ITS区序列的疑似野生“南华”草菇菌株的分子鉴定  

Molecular Identification of Suspected Wild‘Nanhua'Volvariella volvacea Strain Based on Internal Transcribed Spacer Sequence

作　　者：刘主[1] 蔡爱群[1] 刘静华[1] 何智丹 孟晓东[1] 

机构地区：[1]韶关学院英东生命科学学院,广东韶关512005 [2]华南农业大学生命科学学院,广东广州510640

出　　处：《北方园艺》2016年第1期126-130,共5页Northern Horticulture

基　　金：韶关市科技计划资助项目(2013CX/K);韶关学院大学生创新创业训练计划资助项目(Sycxcy2014-028)

摘　　要：以在广东省韶关市"南华寺"周边山岭采得的1株疑似野生"南华"草菇菌株VNH为试材,采用ITS序列克隆和分析,并结合传统形态学研究对其进行分子鉴定。结果表明:菌株VNH为野生"南华"草菇;为进一步研究该野生菌株的生物学特性及驯化栽培研究提供了重要依据;同时,对丰富草菇菌种资源,促进韶关文化和旅游发展具有积极意义。A suspected wild‘Nanhua＇Volvariella volvacea was picked beside the mountains next to Nanhua temple in Shaoguan city of Guangdong Province.The pure culture was obtained by tissue isolation and was named strain VNH.The strain was identified by molecular identification with ITS sequence cloning and analyzing and combine with the traditional morphological.The results showed that,VHN strain was wild‘Nanhua＇V.volvacea.It provided an important basis on the studies of biological characteristics and domestication cultivation for the wild strain.Meanwhile,it had a positive significance to enrich V.volvaceaspecies resources and to promote the development of Shaoguan culture and tourism.

关 键 词：草菇 野生菌株 ITS BLAST 分子鉴定 

分 类 号：S646.13[农业科学—蔬菜学]