生理性深层海水对小鼠高温耐受力的影响  

Effect of physiological deep-sea water on the hyperthermal tolerance of mice

作　　者：代佑果[1] 李为明[2] 唐辉蓉[2] 石安华[3] 甘平[1] 寸英丽[1] 刘琴[1] 关琼瑶[1] 崔进[2] 

机构地区：[1]昆明医科大学第三附属医院/云南省肿瘤医院腹部外科,650118 [2]昆明医科大学第二附属医院干部医科外科,650101 [3]云南中医学院病理教研室,昆明650500

出　　处：《重庆医学》2016年第1期33-36,共4页Chongqing medicine

基　　金：云南省科技厅-昆明医科大学联合专项基金资助项目(2010CD166)

摘　　要：目的探讨生理性深层海水(PDSW)对45.0℃高温环境动物耐受性的影响及机制。方法将取自我国海南省海域的深层海水进行制备,制成PDSW,检测所含有的部分元素。20只昆明小鼠,雌雄各半,分为对照组和实验组,对照组小鼠饮用自来水,实验组饮用PDSW,连续15d,然后把各组小鼠放入45.0℃高温环境下饲养,记录每只小鼠死亡时间,直至各组小鼠全部死亡为止。并取每只小鼠脑、肺、心、肝和肾做病理检查。结果无论是雌性小鼠还是雄性小鼠,实验组的小鼠在45.0℃高温下的生存时间比对照组的小鼠长,差异有统计学意义(P<0.05)。并且雌性小鼠的生存时间要比雄性小鼠的生存时间长,差异有统计学意义(P<0.05)。组织学分析表明PDSW对实验小鼠的脑、心、肺、肝、肾等器官均有保护作用。Western blot检测发现,实验组小鼠肝组织HSP72表达较对照组强,差异有统计学意义(P<0.05)。结论 PDSW可以增强小鼠对45.0℃高温环境的耐受性,其机制考虑与PDSW促进热休克蛋白表达有关。Objective To explore the effects of physiological deep-sea water（PDSW） on hyperthermal tolerance of Kunming （KM） mice in the 45.0 ℃ environment. Methods Deep-sea water from the south Chinese sea was processed,and the metallic elements dissolved in the DSW were analysed. The mice were randomly divided into 2 groups-the control group received tap water; the experimental group treated with PDSW for 15 d. And then the mice were fed in the 45. 0 ℃ eonditiong. The survival time and histomorphometric analyses of the brain, lung, heart, liver and kidney were investigated. Results The survival time in PDSW-fed group was significantly longer than that of the control group （P〈0. 05）. Moreover,histomorphometric analyses showed that PDSW could protect the brain, lung, heart ; liver and kidney of KM mice from the 45.0 ℃ conditions. The results of western blot revealed that expression of H SP72 of liver tissues for PDSW-fed group substantially increased,when compared with the control mice（P〈0.05）. Conclusion PDSW could improve hyperthermal tolerance of KM mice,which maybe in the relation with expression o5 HSP72 promoted by PDSW.

关 键 词：生理性深层海水 高温 诱发 耐受力 

分 类 号：R730.5[医药卫生—肿瘤]