喉鳞状细胞癌组织中碳酸酐酶9与P糖蛋白的表达和临床意义  

Expression and clinical pathological implications of carbonic anhydrase 9 and P glycoprotein in laryngeal squamous cell carcinoma

作　　者：陈歆维[1] 王菲[1] 谢芳[2] 董频[1] 英信江[1] 

机构地区：[1]南京医科大学上海一院临床医学院耳鼻咽喉头颈外科,上海200080 [2]复旦大学附属眼耳鼻喉科医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2016年第1期42-44,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：上海市自然科学基金面上项目(No:13ZR1433600)

摘　　要：目的:研究喉鳞状细胞癌与喉良性病变组织中碳酸酐酶9(CAⅨ)和P糖蛋白(P-gp)表达的差异,分析2种蛋白在喉鳞状细胞癌组织中表达的相关性,及两者与喉鳞状细胞癌临床病理特征的关系。方法:采用免疫组织化学EnVision二步法分别检测47例喉鳞状细胞癌组织、20例声带息肉组织中CAⅨ和P-gp的表达情况。结果:CAⅨ和P-gp在喉鳞状细胞癌组织中的阳性表达率和它们在声带息肉中的表达均差异有统计学意义(P<0.05),且两者在喉鳞状细胞癌组织中的表达呈正相关(r=0.324,P<0.05)。CAⅨ蛋白表达与喉鳞状细胞癌的临床分期、淋巴结转移有关(P<0.05);与喉鳞状细胞癌组织学分级、原发部位无关(P>0.05)。P-gp表达与喉鳞状细胞癌的临床分期、组织学分级有关(P<0.05);与喉鳞状细胞癌的原发部位、淋巴结转移无关(P>0.05)。结论:CAⅨ与P-gp的高表达可能在喉鳞状细胞癌的病情进展中起一定作用。Objective：To identify the difference of CA Ⅸ and P-gp expression level between laryngeal squamous cell carcinoma（LSCC）and benign tissues,evaluate the relationship of these two proteins in LSCC,and their correlation with clinical and pathological features.Method：Immunohistochemical detection of CA Ⅸ and P-gp were performed in 47 cases of LSCC and 20 cases of vocal cord polyps.Result：Overexpression of CA Ⅸand P-gp both in LSCC and in vocal cord polyp（P0.05）were confirmed,with a correlation between the two proteins in LSCC（r=0.324,P0.05）.The expression of CA Ⅸ was related to clinical staging and lymph node metastasis in LSCC（P0.05）.While P-gp was related to clinical staging and histological grading in LSCC（P0.05）.Conclusion：The overexpression of CA Ⅸ and P-gp may play a role in LSCC progression.

关 键 词：喉肿瘤 碳酸酐酶9 P糖蛋白 免疫组织化学 

分 类 号：R739.6[医药卫生—肿瘤]