丙型肝炎病毒相关性肝细胞癌的基因表达分析  

Gene expression analysis of hepatitis C virus related hepatocellular carcinoma genes

作　　者：王凤[1] 孙朝晖[2] 马文丽[1] 郑文岭[1] 

机构地区：[1]南方医科大学基因工程研究所,广州510515 [2]广州军区广州总医院检验科,广州510010

出　　处：《解剖学报》2016年第1期67-71,共5页Acta Anatomica Sinica

基　　金：广东省领军人才基金(C1030925);国家自然科学基金(39880032)

摘　　要：目的分析慢性丙型肝炎发展为肝细胞癌(HCC)过程中的差异表达基因谱。方法以GEO数据库的基因表达谱数据GDS4880、GDS4887为分析材料,采用Qlucore Omics Explorer 3.0软件筛选慢性丙型肝炎与丙型肝炎病毒(HCV)相关性肝细胞癌芯片数据的差异表达基因,结合生物信息学工具PANTHER、DAVID、STRING、Cytoscape对差异表达基因及其相互作用关系进行分析。结果筛选出共差异表达基因328个,其中上调表达133个,下调表达195个。这些差异表达基因主要涉及代谢过程、生物调节、定位等生物过程,p53信号通路、胰岛素信号通路、磷脂酰肌醇信号系统、细胞凋亡等信号通路。差异表达基因编码蛋白间的相互作用主要集中在14个蛋白质(CYP2B6、CYP2E1、AKT1、CDK16、RELA、CDC27、PIK3CA、GNB1、FOXO1、FYN、PDPK1、SCAND1、SGOL1、RPH3AL)。结论 CYP2E1等14个基因可能与HCV相关性肝细胞癌的发生发展相关。Objective To analyze the differentially expressed gene profiling during the development from chronic hepatitis C to hepatocellular carcinoma（HCC）. Methods The research uses the gene expression profile data GDS4880 and GDS4887 of the GEO database as the analysis material to make screening of the differential expression genes of the chronic hepatitis C and the hepatitis C virus（HCV） related HCC＇s microarray data by using Qlucore Omics Explorer 3.0 software, and analyze the differential expression genes and their interaction relations by using the bioinformaties tools PANTHER, DAVID, STRING and Cytoscape. Results 328 differentially-expressed genes were screened out（ 133 with upward effects and 195 with downward effects）. The relevant genes were mainly involved in biological pathways such as metabolic process,biological regulation,localization, p53 signaling pathway, phosphatidylinositol signaling system, insulin signaling pathway, apoptosis, etc. The interaction between encoded proteins of the differential expression gene mainly focuses on the 14 proteins（CYP2B6, CYP2E1, AKT1, CDK16, RELA, CDC27, PIK3CA, GNB1, FOXO1, FYN, PDPK1, SCAND1, SGOL1, RPH3AL） Conclusion The 14 genes may be associated with the development of HCV-related hepatocellular carcinoma.

关 键 词：肝细胞癌 生物信息学 基因表达 Qlueore OMICS EXPLORER软件 

分 类 号：Q786[生物学—分子生物学]