MICA在喉癌组织和细胞中的表达及意义  

Expression and clinical significance of MICA in laryngeal carcinoma tissue and cells

作　　者：王睿[1] 王郡甫[2] 苏庆红[2] 陈雪梅[3] 许晓群[2] 

机构地区：[1]菏泽市立医院神经外科,山东菏泽274000 [2]山东省医学科学院基础医学研究所 [3]山东大学第二医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2016年第2期94-97,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金青年基金(No:81202314);山东省自然科学基金(No:ZR2013HL028;ZR2012HL23和ZR2010CM067);山东省大型科学仪器升级改造技术研究专项(No:2013SJGZ09)

摘　　要：目的:探讨MICA在喉癌细胞Hep-2和喉鳞状细胞癌组织中的表达及意义。方法:RT-PCR和Western-blot方法检测MICA mRNA和蛋白在喉癌细胞和喉癌组织中的表达情况,并以喉外伤组织为对照。结果:RT-PCR检测结果显示MICA在Hep-2细胞中强势表达。与对照相比,MICA在喉癌组织中的表达均有增强(P<0.01),MICA表达强度与临床分期无关。Western-blot检测结果显示MICA蛋白在Hep-2细胞呈强势表达,但在喉癌组织中MICA蛋白随临床分期增高而表达降低。结论:MICA mRNA在Hep-2细胞和喉癌组织中均强势表达,其表达水平与临床分期无关。MICA蛋白在Hep-2细胞呈强势表达,但在喉癌组织中MICA蛋白随临床分期增高而表达降低。由此可见,MICA可能通过多种机制参与喉癌的发生、发展。Objective：To investigate the mRNA and protein expression of MICA in laryngeal squamous cell carcinoma tissue and the Hep-2 cells.Method：Reverse transcriptase-polymerase chain reaction（RT-PCR）and Western-blot were used to detect the expression of MICA mRNA and protein levels in the Hep-2 cells and laryngeal cancer tissues.Result：The MICA mRNA showed higher expression in Hep-2 cells by RT-PCR.Compared with the control,the mRNA expression of MICA was significantly enhanced in laryngeal cancer tissues（t=11.878,P0.01）.The intensity of MICA expression is not related to the clinical stage of cancer.MICA protein demonstrated higher level expression by Western blot.The intensity of MICA protein expression was decreased with increased clinical stage in laryngeal cancer tissues.Conclusion：The MICA mRNA showed stronger expression in Hep-2 cells and laryngeal cancer tissues.The intensity of its expression is not related to clinical stage of cancer.The MICA protein expression was strong in Hep-2 cells.The intensity of MICA protein expression was decreased with increased clinical stage in laryngeal cancer tissues.MICA may play an important role in laryngeal carcinoma process.

关 键 词：MICA HEP-2细胞 喉肿瘤 

分 类 号：R739.65[医药卫生—肿瘤]