内源性促红细胞生成素及促红细胞生成素受体mRNA与新生大鼠脑白质损伤的相关性  

The correlation between endogenous EPO/EPOR m RNA and the white matter damage in brain tissue of neonatal rats

作　　者：刘燕[1] 姜红[1] 王倩[1] 

机构地区：[1]青岛大学附属医院,山东青岛266003

出　　处：《临床儿科杂志》2016年第1期46-49,共4页Journal of Clinical Pediatrics

基　　金：山东省科技发展计划项目(NO.211YD18084)

摘　　要：目的探讨新生大鼠脑组织内源性促红细胞生成素（EPO）mRNA及EPO受体（EPOR）mRNA水平的变化与脑白质损伤的相关性。方法10只孕15dWistar大鼠，5只腹腔注射脂多糖（300μg／kg）制作宫内炎症致脑白质病变的新生鼠动物模型，另5只腹腔注射等量生理盐水作为对照；孕鼠分娩后各选取18只新生鼠作为实验组和对照组。苏木精一伊红染色评价脑白质损伤情况，实时荧光定量PCR方法检测胼胝体内源性EPO及EPORmRNA水平，免疫荧光法检测脑室周围白质CD68、胶原纤维酸性蛋白（GFAP）、髓鞘碱性蛋白（MBP）水平，比较两组间各指标的差异，以及CD68、GFAP及MBP与EPOmRNA及EPORmRNA水平的相关性。结果实验组新生鼠脑白质结构稀疏，呈网状改变，脑白质损伤明显；实验组CD68、GFAP及胼胝体EPOmRNA、EPORmRNA水平均明显高于对照组，差异有统计学意义（P均〈0．01）。Pearson相关性分析显示，CD68、GFAP与胼胝体内源性EPO及EPORmRNA水平呈显著正相关（r=0．92-0．95，P均〈0．01）。结论宫内炎症致脑白质损伤新生鼠内源性EPOR表达增加，可能是EPO对感染所致脑损伤保护作用的基础。Objective To investigate the correlation between the level of endogenous erythropoietin （EPO） mRNA/EPO receptor （EPOR） mRNA and the white matter damage in brain tissue of neonatal rats. Methods Ten pregnant 15 days Wistar rats, 5 were intraperitoneally injected LPS （300μg/kg） to make model of cerebral white matter lesions caused by intrauterine inflammation and 5 were intraperitoneally injected same amount of normal saline as control. After the pregnant rats giving birth, 18 newborn rats were selected from experimental group and control group respectively. Cerebral white matter damage in newborn rats was detected by hematoxylin eosin （HE） staining. The level of EPO and EPOR mRNA in the corpus caUosum were detected by real time fuorescent quantitative polymerase chain reaction. The levels of CD68, glial fibrillary acidic protein （GFAP）, myelin basic protein （MBP） in periventricular white matter were detected by immunofluorescence. The differences between two groups were compared. The correlation of CD68, GFAP, and MBP with MBP, EPO mRNA, and EPOR mRNA, were analyzed. Re- suits In experimental group, the structure of periventricular white matter of newborn rats was scattered, with reticular change. The white matter damage was apparent. The levels of CD68, GFAP, EPO mRNA and EPOR mRNA in experimental group were significantly higher than those in control group （P〈0.01）. Pearson correlation analysis showed that the levels of CD68 and GFAP were positively correlated with the levels of endogenous EPO and EPOR mRNA （r=0.92-0.95, all P〈0.01）. Conclusion The expression of endogenous EPOR mRNA rises in white matter damage caused by intrauterine infection in newborn rats, which may be the basis of the protective effect of EPO in brain damage.

关 键 词：促红细胞生成素 促红细胞生成素受体 脑白质 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]