冷驯化对中缅树鼩褐色脂肪组织适应性产热的影响  

Adaptive thermogenesis of the brown adipose tissue in tree shrews( Tupaia belangeri) during cold acclimation

作　　者：高文荣[1,2] 曹能[1] 朱万龙[1] 张浩[1] 王政昆[1] 陈金龙 

机构地区：[1]云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室,云南师范大学生命科学学院,昆明650500 [2]云南师范大学能源与环境科学学院,昆明650500 [3]昆明市海口林场,昆明650114

出　　处：《中国实验动物学报》2015年第6期567-572,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家科技支撑计划项目子课题(编号:2014BAI01B01-06);国家国际科技合作专项项目(编号:2014DFR31040);国家自然科学基金项目(编号:No.31360096);云南省博士研究生学术新人奖资助

摘　　要：目的探讨环境温度对中缅树鼩体重、褐色脂肪组织(BAT)产热活性及解偶联蛋白1含量的影响,为建立树鼩肥胖模型提供理论依据。方法将40只体重相似的成年中缅树鼩随机分为5组(每组8只):对照组(0 d),置于(25±1)℃,12 L/12 D条件下饲养;以及置于(5±1)℃,12 L/12 D条件下分别驯化7、14、21 d和28d组。驯化结束后测定动物的体重、非颤抖性产热(NST)、褐色脂肪组织重量以及解偶联联蛋白1(UCP1)的含量。结果与对照组(0 d)相比,冷驯化组中缅树鼩的体重、NST、BAT重量以及UCP1含量都显著增加,BAT颜色也明显加深,冷驯化28 d后,体重增加了26.32%,NST增加了20.65%,BAT重量增加了53.85%,UCP1含量增加了43%。UCP1含量与BAT重量和NST显著正相关。结论中缅树鼩可能通过冷驯化诱导BAT组织增生和UCP1表达上调,从而增强BAT产热活力以增加能量支出,推测BAT可能作为以能量学途径治疗肥胖的靶器官。Objective To investigate the effect of ambient temperature on body mass,thermogenic activity and uncoupling protein-1（ UCP1） content of brown adipose tissue（ BAT） in tree shrews（ Tupaia belangeri）,and to provide theoretical basis for establishing tree shrews model of obesity. Methods Forty healthy adult tree shrews with similar body mass were uesd in our experiment. The tree shrews were divided into five groups（ n = 8） ： control group（ 0 d）,the animals were maintained under 25 ± 1℃ and 12L： 12D（ light ： dark,lights on 08： 00） photoperiod; and the animals were maintained under 5 ± 1℃ and 12L： 12 D photoperiod for 7 d,14 d,21 d and 28 d groups,respectively. At the end of experiment,the changes of body mass,nonshivering thermogenesis（ NST）,BAT mass and uncoupling protein 1（ UCP1） content were determined. Results Compared with the control group（ 0 d）,the body mass,NST,BAT mass and UCP1 content of the cold acclimation groups were improved significantly,the BAT color also obviously deepened,and after cold acclimation for 28 d,the body mass,NST,BAT mass and UCP1 content were increased by 26. 32%,20. 65,53. 85% and 43%,respectively. Apparently,the UCP1 content was significantly positively correlated with BAT mass and NST. Conclusions BAT proliferation may be induced and UCP1 expression upregulated by cold acclimation in Tupaia belangeri,therefore,en-hancing the thermogenic activity of brown adipose tissue to increase energy expenditure. We would speculate that BAT might be used as a target organ for treatment of obesity by energetic approach in the future.

关 键 词：中缅树鼩 冷驯化 非颤抖性产热 褐色脂肪组织 解偶联蛋白1 肥胖 产热 

分 类 号：Q95-33[生物学—动物学]