血栓通胶囊抗急性心肌缺血和抗血栓形成的作用  

Protective effect of Xueshuantong capsule on acute myocardial ischemic injury and its antithrombotic effect in rats

作　　者：武汀[1] 张硕峰[1] 董世芬[1] 吴金英[1] 贾占红[1] 张胜威[1] 张荣[1] 梁耀月 孙建宁[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国比较医学杂志》2015年第12期10-14,25,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然基金(编号:81503287);北京市自然基金(编号:7144222);北京中医药大学校级自助课题(编号:2013-JYBZZ-XS-073)

摘　　要：目的研究血栓通胶囊对冠脉结扎致急性心肌缺血模型大鼠的保护及抗血栓形成的作用。方法采用结扎左冠状动脉前降支法复制急性心肌缺血大鼠模型,观察血栓通胶囊对各组大鼠心电图、心肌梗死范围以及乳酸脱氢酶(lactate dehydrogenase,LDH)、肌酸激酶(creatine kinase,CK)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、α-羟丁酸脱氢酶(α-hydroxybutyrate dehydrogenase,α-HBDH)、肌酸激酶同工酶MB(creatine kinase-MB,CK-MB)的影响;采用体外方法,观察血栓通胶囊对血栓形成及血小板聚集的影响。结果血栓通胶囊60、30、15 mg/kg组可显著减少心肌缺血模型大鼠心肌梗死范围,降低血清LDH、CK及CK-MB水平;其中血栓通胶囊60 mg/kg组对心电图异常发生百分率有明显抑制作用。血栓通胶囊30、15 mg/kg可显著促进体外血栓溶解;血栓通胶囊60、30、15 mg/kg可显著抑制体外ADP、胶原诱导血小板聚集。结论血栓通胶囊对大鼠急性心肌缺血引起的损伤有保护作用,可能与抗血栓形成有关。Objective To explore the protective effect of Xueshuantong capsule on acute myocardial ischemic injury and its antithrombotic effect in rats. Methods The rat model of acute myocardial ischemia（ AMI） was developed by ligation of the left anterior descending coronary artery for 24 hours. The effects of Xueshuantong capsule,a drug of traditional Chinese medicine,on the electrocardiogram（ ECG） and myocardial infarct size was observed. The serum activities of lactate dehydrogenase（ LDH）,creatine kinase（ CK）,aspartate aminotransferase（ AST）,creatine kinase-MB（ CK-MB）,and α-hydroxybutyrate dehydrogenase（ α-HBDH） were detected by biochemistry. Blood was taken from the rats,and the thrombolytic effect and the platelet aggregation rate in vitro were observed. Results Xueshuantong capsule in each dose（ 60,30,15 mg / kg） group significantly reduced the myocardial infarct size,and lowered the serum contents of CK,LDH,and CK-MB. Xueshuantong capsule in a dose of 30 and 15 mg / kg significantly promoted the thrombolysis in vitro,and Xueshuantong capsule in a dose of 60,30 and 15 mg / kg significantly inhibited the ADP-and collagen-induced platelet aggregation in vitro. Conclusions Xueshuantong capsule exerts protective effects on acute myocardial ischemic injury in rats. This effect may be partly associated with its antithrombotic effect.

关 键 词：血栓通胶囊 三七总皂苷 心肌缺血 心肌梗死 心电图 抗血栓作用 大鼠 

分 类 号：R-33[医药卫生]