17β雌二醇对丙泊酚诱导皮层神经元凋亡的影响  

Effects of 17β-estradiol on propofol-induced rat cortical neuroapoptosis

作　　者：李建立[1] 郭洪霞[1] 梁巍[2] 吴红海[3] 侯艳宁[3] 

机构地区：[1]河北省人民医院麻醉科,石家庄河北050051 [2]河北省人民医院普外科,石家庄河北050051 [3]白求恩国际和平医院药剂科,石家庄河北050082

出　　处：《中国比较医学杂志》2015年第12期32-36,I0002,共6页Chinese Journal of Comparative Medicine

基　　金：2014年河北省卫生厅指令性课题(编号:ZL20140095)

摘　　要：目的探讨17β雌二醇对丙泊酚诱导原代培养皮层神经元凋亡的影响及机制。方法原代培养7d的大鼠皮层神经元,随机分为三组:溶剂对照组(给予等溶剂20%脂肪乳),丙泊酚组(丙泊酚终浓度为500μmol/L),17β雌二醇+丙泊酚组(17β雌二醇终浓度为0.1μmol/L,丙泊酚终浓度为500μmol/L)。上述药物处理皮层神经元12 h后,用Hoechst 33258核染色法和TUNEL法检测皮层神经元调亡,Western-blot法测定神经元Bcl-2,Bax和cleaved caspase-3蛋白的水平。结果与溶剂对照组比较,丙泊酚组神经元凋亡率显著性增加(P<0.01),Bcl-2蛋白水平显著性下降(P<0.01),Bax蛋白水平显著性增加(P<0.01),Bcl-2/Bax显著性降低(P<0.01),cleaved caspase-3蛋白水平显著性增加(P<0.01)。与丙泊酚组比较,17β雌二醇+丙泊酚组神经元凋亡率显著性下降(P<0.01),Bcl-2蛋白水平显著性增加(P<0.01),Bax蛋白水平显著性下降(P<0.01),Bcl-2/Bax显著性增加(P<0.01),cleaved caspase-3蛋白水平显著性下降(P<0.01)。结论 17β雌二醇可通过影响Bc1-2和Bax蛋白水平抑制皮层神经元凋亡,产生保护作用。Objective To investigate the protective effects and the mechanisms of 17β-estradiol on the propofolinduced neuroapoptosis in primary cultured rat cortical neurons. Methods The neurons were cultured for 7 days and then divided into three groups： vehicle-control group（ treated with equal volume of 20% intralipid）,propofol-treated group（ treated with 500 μmol/L propofol）,and propofol plus 17β-estradiol treated group（ treated with 500 μmol/L propofol and0. 1 μmol / L 17β-estradiol）. 12 hours after the treatment,neuroapoptosis was detected by Hoechst 33258 staining and TUNEL assay,and the levels of Bcl-2,Bax and cleaved caspase-3 proteins were detected by Western blot. Results Compared with the vehicle-control group,the neuroapoptosis increased greatly（ P〈0. 01）,Bcl-2 level reduced（ P〈0. 01）,Bax and cleaved caspase-3 levels increased greatly（ P〈0. 01）,and Bcl-2 / Bax ratio reduced significantly（ P〈0. 01）. Compared with the propofol-treatment group,the neuroapoptosis decreased greatly（ P〈0. 01）,Bcl-2 level increased（ P〈0. 01）,Bax and cleaved caspase-3 levels reduced greatly（ P〈0. 01）,and Bcl-2 / Bax ratio increased greatly（ P〈0. 01）. Conclusions 17β-estradiol can protect cortical neurons against propofol-induced cortical neuroapoptosis by regulating the expression of Bcl-2 and Bax.

关 键 词：丙泊酚 17Β雌二醇 原代培养皮层神经元 凋亡 

分 类 号：R-33[医药卫生]