电刺激点燃癫痫大鼠的皮层脑电及行为学研究  

Electrocorticogram and behavior of electrically kindled epileptic rats

作　　者：张瑞华[1] 王玉平[2] 

机构地区：[1]首都医科大学附属北京潞河医院,北京101149 [2]首都医科大学宣武医院,北京100053

出　　处：《中国比较医学杂志》2015年第12期54-58,共5页Chinese Journal of Comparative Medicine

摘　　要：目的应用Sprague-Dawley（SD）大鼠建立难治性癫痫杏仁核电刺激点燃癫痫模型,观察电刺激点燃模型的皮层脑电变化。方法用雄性SD大鼠,将双极电极立体定向植入大鼠右侧杏仁核,术后2周测定大鼠后放电阈值（after discharge threshold,ADT）,用快速点燃方案建立杏仁核电刺激点燃癫痫模型。2周后再次点燃。应用皮层脑电图（electrocorticogram,ECoG）记录动物皮层脑电变化,应用视频监测仪记录大鼠的行为学改变。结果大鼠在测ADT过程中表现1~3级发作,ECoG显示异常放电;电刺激点燃时大鼠首先表现出1~3级发作,发作程度逐渐加剧直到出现4~5级发作,脑电图显示棘波、尖波、棘-慢波和尖-慢波等。统计学结果：首次点燃ADT：（82.33±21.29）μA,2周后ADT：（83.17±15.76）μA,P=0.923,差异无统计学意义;首次点燃电刺激点燃动物出现第一个4级发作所需要的电脉冲串的个数：（4.41±2.27）,2周后：（5.58±3.96）,P=0.231,差异无统计学意义。结论快速点燃癫痫动物模型是一个有效的癫痫模型,其稳定性在2周内持续存在。Objective The current study was aimed to establish an electrically amygdala kindling model of refractory epilepsy in Sprague-Dawley rats,and to study their changes of electrocorticagram（ ECoG）. Materials and methods Male Sprague-Dawley rats were used in the experiment. Two-polar electrode was implanted into the right amygdala stereotactically. Two weeks after the surgery,electrical stimulations were given to elicit the grade 4 seizure in all animals with the fast kindling protocol. Rekindling was administrated after the first kindling. The after discharge threshold（ ADT） and the number of stimulus were reassessed in the two kindling protocols. ECoG and the behavior of the animals were recorded during the whole experiment. Result The changes of ECoG and behavior： Animals showed stage 1-3seizure when the ADT was assessed. While during the kindling period,the animals showed generalized convulsion with stage 4-5 seizure. ECo G showed sharp waves,spike waves,sharp-slow waves and spike-slow waves. Statistical analysis showed that the ADT was not significantly increased at two weeks after kindling（ from 83. 33 ± 22. 29 μA to 84. 17 ± 16. 76μA,P = 0. 923）. The number of stimulus given to elicit the stage 4 convulsion was not significantly increased as well（from 4. 41 ± 2. 27 to 5. 58 ± 3. 96,P = 0. 231）. Conclusions Rapid kindling model of epilepsy in rats is an effective epilepsy model,which is stable for 2 weeks.

关 键 词：电刺激点燃 难治性癫痫 杏仁核点燃 皮层脑电图 大鼠 

分 类 号：R-33[医药卫生]