大观霉素间接竞争ELISA的建立  

Development of an Indirect Competitive ELISA for Determination of Spectinomycin in Animal-origin Food

作　　者：王文珺 高隆轩 米振杰 吴雨洋 王照鹏 苏丽芳 韩霄[4] 邢佑尚 

机构地区：[1]北京维德维康生物技术有限公司,北京100095 [2]中国农业大学动物医学院,北京100193 [3]河北兽药监察所,河北石家庄050035 [4]北京交通大学理学院,北京100044

出　　处：《动物医学进展》2016年第1期43-46,共4页Progress In Veterinary Medicine

基　　金：国家"十二五"科技支撑计划项目(2012BAK17B16);河北省科技计划项目(15277107D)

摘　　要：本研究的目的是建立大观霉素的间接竞争性ELISA（icELISA）,用于动物源性食品的检测。在优化的条件下,建立了大观霉素icELISA的标准曲线,半抑制浓度IC50=1.78ng/mL。多克隆抗体对大观霉素的特异性较强,与其他氨基糖苷类化合物有微小的交叉。分别对20份空白的牛奶、猪肉、鸡肉进行检测,确定这些样本中大观霉素的检测限分别是4.8、9.3、10.0μg/kg。在样本中添加不同浓度的大观霉素,添加回收率的范围为77.1%~116.0%,变异系数为4.6%~9.7%;ELISA和GC/MS两种方法分析结果无显著性差异（P〉0.05）。结果表明,本研究成功建立了大观霉素的icELISA,用于检测牛奶、猪肉、鸡肉中大观霉素,准确度和精密度均满足兽药残留检测的要求。The objective of this work was to establish an indirect competitive ELISA for the determination of spectinomycin in animal-origin food.Under optimized condition,the half maximal inhibitory concentration（IC50）is 1.78ng/mL.The limit of detection for milk,pork and chicken are 4.8μg/L,9.3μg/kg and 10.0μg/kg,respectively.The samples were spiked with spectinomycin and the recoveries were 77.1%-116.0%;the coefficients of variation were 4.6%-9.7%.There were no significant differences between the results of ELISA and GC/MS（P〉0.05）.The results showed an indirect competitive ELISA for spectinomycin was established successfully and this method was primarily applied to detect spectinomycin in animal-origin food.

关 键 词：大观霉素 间接竞争ELISA 动物性食品 

分 类 号：S859.796[农业科学—临床兽医学]