P2Y6受体在慢性癫痫发作大鼠海马组织中的表达及对Ca^(2+)的影响  

P2Y6Expression in Rat Model of Temporal Lobe Epileptic and Its Effects on Intracellular Ca^(2+)

作　　者：刘艳 宋鹏辉[2] 甘学军 熊瑛 邓斌 康慧聪[3] 

机构地区：[1]武汉市第八医院神经内科,湖北武汉430010 [2]华中科技大学同济医学院附属协和医院神经内科,湖北武汉430022 [3]华中科技大学同济医学院附属同济医院神经内科,湖北武汉430030

出　　处：《武汉大学学报（医学版）》2016年第1期10-14,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金青年项目(编号:81201006);武汉市卫计委临床医学科研项目(编号:WX14A09)

摘　　要：目的:建立慢性癫痫发作SD大鼠模型并检测海马组织P2Y6受体表达改变,研究P2Y6受体-Ca2+信号通路改变。方法:采用氯化锂和匹罗卡品腹腔注射建立SD大鼠慢性癫痫发作模型(EPI组)并检测其脑电图,以腹腔注射生理盐水SD大鼠为对照(CON组)。采用免疫组织化学、RT-PCR和Western blot检测EPI组和CON组SD大鼠海马组织P2Y6受体mRNA和蛋白质表达并比较其差异。采用Fura-2/AM检测EPI组和CON组SD大鼠新鲜海马组织细胞内Ca2+浓度并对比其差异。结果:CON组正常SD大鼠脑电图波形主要以α波为主,并伴有低幅度的β波及少量的δ波,几乎未捕捉到棘波发放;EPI组SD大鼠的脑电图出现较为规律的棘波发放,其频率多集中于28Hz,波幅不高于200μV。EPI组慢性癫痫发作SD大鼠海马组织P2Y6受体表达、mRNA、蛋白质和细胞内Ca2+显著高于CON组正常SD大鼠(均P<0.05)。结论:P2Y6受体在慢性癫痫发作大鼠海马组织表达显著升高,激活下游信号传导通路导致细胞内Ca2+水平升高,可能在慢性癫痫发作的发病机制中具有重要作用。Objective：To detect P2Y6receptor＇s expressions in rat model of temporal lobe epileptic and study its effects on intracellular Ca^2＋. Methods：Lithium-pilocarpine was used to establish the rat model of temporal lobe epileptic（EPI group）,while the rat with intraperitoneally infused with saline was as control group（CON group）.The P2Y6 expressions and intracellular Ca^（2＋）between two groups were compared.Results：The rat model of temporal lobe epileptic was successfully es-tablished with Lithium-pilocarpine method.The P2Y6 expressions and intracellular Ca^2＋ in EPI group were significantly higher than in CON group（P〈0.05）.Conclusion：P2Y6＇s expression in rat model of temporal lobe epileptic increases significantly,and promotes intracellular Ca^（2＋）,which may play an important role in epilepsy＇s pathogenesis.

关 键 词：P2Y6受体 SD大鼠 慢性癫痫发作 细胞内CA2+ 

分 类 号：R741.02[医药卫生—神经病学与精神病学]