齐墩果酸对阻塞性胆汁淤积大鼠胆酸转运蛋白OSTα/β和BSEP的调控作用  

Oleanolic acid stimulates the expression of bile acids transporters OSTalpha-beta and BSEP in bile duct-ligation rats

作　　者：柴进[1] 向国春[2] 封欣婵 张樑君 程英[1] 陈文生[1] 

机构地区：[1]第三军医大学西南医院全军消化病研究所,重庆400038 [2]第三军医大学西南医院临床教学管理中心,重庆400038

出　　处：《局解手术学杂志》2016年第1期1-4,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然基金青年项目(81100280);国家自然基金面上项目(81470880)

摘　　要：目的建立胆道结扎大鼠模型,观察胆道结扎所致肝外胆汁淤积时齐墩果酸对肝细胞表面胆酸转运蛋白OSTα/β和BSEP的调控作用。方法随机将45只SD大鼠分为9组,每组5只。假结扎组(Sham组)、胆道结扎组(BDL组)和齐墩果酸组(BDL+OA组)分别用生理盐水、齐墩果酸处理3 d、7 d、14 d后收集组织样本,提取肝脏RNA和总蛋白,分别在mRNA水平和蛋白水平检测OSTα/β和BSEP的表达变化。结果大鼠胆道结扎后,齐墩果酸干预3 d,胆酸转运蛋白OSTβ在mRNA和蛋白水平均增高,随着齐墩果酸干预时间的延长,在第7天时,胆酸转运蛋白BSEP的表达亦出现增高。第14天时,OSTα、OSTβ、BSEP表达均增高。结论齐墩果酸能够上调阻塞性胆汁淤积胆酸转运蛋白OSTα、OSTβ、BSEP的表达。Objective To study the function and effect of Oleanolic acid to cholestasis on regulating bile acids transporters. Methods A total of 45 rats were divided into 9 groups,5 rats in each group. The Sham group,Bile duct ligation group and Oleanolic acid group were treated with saline or Oleanolic acid for 3 days,7 days and 14 days. Then the liver and tested bile acids transporters in mRNA and protein levels were collected. Results The expression of bile acids transporter OSTβ with Oleanolic acid was increased after 3 days. The expression of OSTβ and BESP in Oleanolic acid group after 7 days were increased than those in Sham group. Fourteen days later,the increasing tendency of OSTα,OSTβ and BSEP were sensiable. Conclusion Oleanolic acid can stimulates the expression of bile acids transporters OSTalpha-beta and BSEP in bile duct-ligation rats.

关 键 词：胆汁淤积 齐墩果酸 胆酸转运蛋白 胆道结扎 

分 类 号：R735.37[医药卫生—肿瘤]