解淀粉芽孢杆菌CMN1308抗真菌特性研究  

Characterizing a Novel Strain of Bacillus amyloliquefaciens CMN1308 for Potential Biological Control in Pathogenic Bacterium

作　　者：张雪花[1,2] 李琳玲[1] 程华[1] 艾远航 王晖[1] 程水源[2,3] 

机构地区：[1]黄冈师范学院经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000 [2]长江大学园艺园林学院,湖北荆州434023 [3]武汉轻工大学生物与制药工程学院,湖北武汉430023

出　　处：《北方园艺》2016年第2期117-121,共5页Northern Horticulture

基　　金：湖北省教育厅中青年基金资助项目(Q20132909);湖北省教育厅高校产学研合作资助重点资助项目(C2010060);黄冈师范学院产学研合作资助项目(2012025903);湖北省自然科学基金重点资助项目(2010CBB03901)

摘　　要：以解淀粉芽孢杆菌CMN1308为试材,采用平板对峙培养法,测定CMN1308对葡枝根霉(Rhizopus stolonifer)、腐皮镰刀菌(Fusarium solani)、黑葡萄穗霉(Stachybotrys chartarum)、栗疫菌(Cryphonectria parasitica)、可可毛色二孢菌(Lasiodiplodia theobromae)、扩展青霉(Penicillium expansum)、黑曲霉(Aspergillus niger)的抑菌效果,并进行了镜检观测及无菌滤液中抑菌成分稳定性测定,以充分研究其抑菌效果及抗真菌特性。结果表明:拮抗菌CMN1308对上述7种供试病原菌均有较强的抑制作用,表现出抑菌谱广的特点;其中对扩展青霉的抑制作用最好,抑菌圈直径可达27.1mm;CMN1308对可可毛色二孢菌菌丝生长有较强的抑制作用,且其发酵产生的抑菌物质热稳定性较好,耐碱怕酸,对紫外线不敏感。这为更进一步探索板栗采后生防保鲜技术和微生物制剂的开发与应用提供了一定的理论与技术支持。Taking Bacillus amyloliquefaciens CMN1308 as materials,with the method of confront culture,the antibiosis effect on Rhizopus stolonifer,Fusarium solani,Stachybotrys chartarum,Cryphonectria parasitica,Lasiodiplodia theobromae,Penicillium expansumand Aspergillus niger was studied.The results showed that CMN1308 had stronger antibiosis effect on the seven major pathogens of chestnut.Among this,CMN1308 had a best biocontrol to P.expansum,the inhibition zone diameter was 27.1mm.The further study on the stability of antimicrobial components in CMN1308 showed,the growth of P.expansumhyphal was limited by the antimicrobial components isolated from CMN1308,which also had a good stability to hot temperature,alkali and UV,but sensitive to acid.The results suggested that antibiotic effect and inducible resistance were the major mechanism of CMN1308 on chestnut pathogenic.The finding of this study would help to optimize the practical use of CMN1308 in the biological control of chestnut or other fruit rot caused by pathogenic fungi.

关 键 词：板栗 生物防治 抗真菌谱 稳定性 

分 类 号：Q946[生物学—植物学]