大鼠牙髓神经胞体在三叉神经节内分布特点  

The neuronal soma trait of trigeminal nerve branch projecting to dental pulp in rat trigeminal ganglia

作　　者：肖芳莉 李国超[1] 马腾飞[1] 黄姗姗[1] 徐文华[1] 王烈成[2] 王元银[1] 

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室,合肥230032 [2]安徽医科大学基础医学院生理学教研室,合肥230032

出　　处：《安徽医科大学学报》2016年第1期64-68,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81271162);安徽省自然科学基金(编号:11040606M204);安徽省高等学校省级优秀青年人才基金(编号:2012SQRL070)

摘　　要：目的了解正常及三叉神经痛(TN)模型大鼠的牙髓神经胞体在三叉神经节(TG)内分布情况及其投射关系,为临床通过TG治疗TN提供解剖学依据。方法本实验分为模型组和假手术组,在两组大鼠不同牙髓腔内注入逆行荧光探针,用荧光显微镜观察TG中荧光标记神经元的分布情况。结果两组大鼠分别在右下及左上中切牙髓腔内注入绿色荧光探针Fast Di O 72 h后,分别在右侧TG后外侧区、左侧TG中段区观察到数个、成簇分布带有绿色荧光标记神经元;将两组大鼠右下及左上中切牙髓腔内分别注入Fast Di O、Fast Di I两种荧光探针,72 h后在其同侧TG的后外侧区和中间区分别观察到成簇分布的带有绿色和红色荧光的神经元;两组大鼠左侧上中切牙及下颌第一磨牙髓腔内分别注入荧光探针Fast Di I和Fast Di O,72 h后在同侧TG的后外侧区和中段区分别观察到成簇分布带有绿色和红色荧光的神经元。经统计学比较,两组之间相应牙髓神经胞体在TG内分布区域、数量及荧光强度没有明显区别。结论三叉神经上、下颌支神经胞体投射于同侧TG,并有一定区域性;TN产生不改变TG内神经元数量及投射联系,其可能与三叉神经功能活动改变有关。Objective To observe the position relationship of neuronal somas in trigeminal ganglia （TG）, which projects from different dental pulps in the normal and trigeminal neuralgia（TN） model rats, and provides anatomi- cal theoretical guidance for the clinical treatment of TN. Methods Those experiments were divided into model group and control group. The retrograde fluorescent probes were injected into the different dental pulp cavities of rats in the two groups, and fluorescent microscope was used to observe distribution of fluorescence labeled neurons in TG. Results Green fluorescent probes Fast DiO were injected into right lower and left upper central incisor of rats in the two groups respectively, a few fluorescence labeled neuronal somas after 72 h could be detected in the posterolateral and the middle area of the ipsilateral TG, which distributed in clusters. Two different fluorescent probes Fast DiO and Fast DiI were injected respectively into right lower and left upper central incisor of rats in the two groups, green fluorescence labeled neuronal somas and red fluorescence labeled neuronal somas in cluster could be detected after 72 h in the posterolateral and the middle area of the ipsilateral TG of the two groups corresponding- ly. Fast DiI and Fast DiO were injected respectively into left upper central incisor and first molar in lower mandibu- lar of rats in the two groups at the same time, green fluorescence labeled neuronal somas and red fluorescence la- beled neuronal somas in cluster could be detected after 72 h in the middle area and the postermiddle area of left TG correspondingly. There was no statistically significant difference of labeled neuronal somas distribution, quantity and fluorescence intensity in TG between the two groups. Conclusion The neuronal somas of trigeminal nerve branches regional distributions on the ipsilateral side TG. The TN does not affect the number of neurons in TG and the projection. It may be associated with the change of trigeminal nerve function.

关 键 词：三叉神经节 三叉神经痛 逆行荧光标记 神经束路追踪法 

分 类 号：R745.11[医药卫生—神经病学与精神病学]