壳聚糖及其水溶性衍生物对小鼠免疫功能的影响  

Effect of Chitosan and Its Water-Soluble Derivatives on Immune Function in Mice

作　　者：王志华[1] 江阳阳 余晓华[1] 颜永斌[2] 覃彩芹[2] 

机构地区：[1]湖北工程学院特色果蔬质量安全控制湖北省重点实验室,湖北孝感432000 [2]湖北工程学院生物质资源化学与环境生物技术湖北省重点实验室,湖北孝感432000

出　　处：《食品科学》2016年第1期198-202,共5页Food Science

基　　金：湖北省教育厅科学技术研究项目(B2015037);国家自然科学基金面上项目(31371750)

摘　　要：给小鼠喂食壳聚糖(chitosan,CS)、羧甲基壳聚糖(carboxymethyl chitosan,CMCS)、壳聚糖季铵盐(quaternary ammonium salt of chitosan,HACC)和羟丙基壳聚糖(hydroxypropyl chitosan,HPCS)30 d后,测定其免疫器官指数、碳粒廓清速率、迟发型变态反应、血清溶血素含量(半数溶血值HC_(50))。结果表明:摄食CS及其水溶性衍生物的小鼠以上指标均高于对照组;除胸腺指数和双耳质量差值外,HACC组小鼠其他各项免疫指标与对照组相比均呈显著或极显著差异(P<0.05或P<0.01);同样,除胸腺指数外,HPCS组小鼠其他各项免疫指标均显著或极显著高于对照组(P<0.05或P<0.01);CMCS组小鼠的吞噬指数(K)和半数溶血值(HC_(50))与对照组相比均呈显著差异(P<0.05);壳聚糖及其3种水溶性衍生物都能够增强小鼠免疫功能,增强效果排序为HPCS>HACC>CMCS>CS,这可能与引入的化学基团不同有关。The immune organ index, carbon clearance rate, delayed type hypersensitivity, and serum hemolysin（HC_（50）） in mice were compared and analyzed after the administration of chitosan（CS）, carboxymethyl chitosan（CMCS）, quaternary ammonium salt of chitosan（HACC） and hydroxypropyl chitosan（HPCS） for 30 days. The values of all investigated parameters were higher in mice administered with CS or its water-soluble derivatives compared with the control group. A significant difference（P 0.05） or extremely significant difference（P 0.01） in all immune indexes except for thymus index and binaural weight difference was found for the HACC and HPCS groups compared with the control group. Comparing the CMCS group with the control group, a significant difference in carbon clearance rate and the content of serum hemolysin（P 0.05） was observed. These results indicated that the administration of chitosan and its water-soluble derivatives could enhance the immune function of mice in the decreasing order of HPCS HACC CMCS CS. This may be associated with the introduction of chemical groups.

关 键 词：壳聚糖 水溶性衍生物 免疫功能 

分 类 号：O636.1[理学—高分子化学]