miRNA-200c在IIIc期卵巢浆液性囊腺癌中的表达及其参与癌转移中的作用  

MiRNA-200c expression in stage IIIc ovarian serous adenocarcinoma and its role in tumor metastasis

作　　者：施君[1,2] 叶婧[1,2] 周舟[3] 周星辰[1,2] 狄文[1,2] 

机构地区：[1]上海交通大学医学院附属仁济医院妇产科,上海200127 [2]上海市妇科肿瘤重点实验室,上海200127 [3]上海市免疫学研究所,上海200025

出　　处：《现代免疫学》2016年第1期36-41,共6页Current Immunology

基　　金：上海市卫生和计划生育委员会项目(2011337)

摘　　要：研究miR-200c在IIIc期卵巢浆液性囊腺癌中的表达格局,探索其参与转移癌形成的相关机制。选取2010年1月至2013年12月上海交通大学医学院附属仁济医院妇科卵巢肿瘤组织标本(IIIc期卵巢浆液性囊腺癌40例(其中选择自身配对的原发灶40例、转移灶40例)、卵巢浆液性囊腺瘤30例为对照),采用real time-qPCR法检测miR-200c的表达;采用CCK8法、Transwell小室分别检测细胞过表达miR-200c后增殖、迁移和侵袭能力的改变;采用western blot法检测相关蛋白表达量。结果发现:(1)IIIc期卵巢浆液性囊腺癌转移灶肿瘤与原发灶肿瘤相比,miR-200c表达是升高的(490.37±78.32cf.157.46±17.21),P<0.01;而且这两者均高于对照组(卵巢浆液性囊腺瘤)的表达(25.32±10.17),P<0.01。SKOV3细胞过表达miRNA-200c后(2)细胞迁移能力降低(35.6±4.3cf.18.6±1.9),P<0.05;侵袭能力降低(26.7±3.1cf.15.4±2.4)P<0.05;但是增殖能力不改变。(3)在蛋白水平检测ZEB1蛋白表达下降(0.3023±0.0251cf.0.6753±0.0262)P<0.05、E-cad蛋白表达升高(1.274±0.0331cf.0.6140±0.0460)P<0.05、Vimentin蛋白表达降低(0.0957±0.0049cf.0.1767±0.0158)P<0.05。可见,miR-200c在IIIc期卵巢浆液性囊腺癌组织样本中表达增高,检测miR-200c的表达格局对于临床上监测上皮性卵巢癌的临床进展和转移有重要意义;也为开拓过表达miR-200c作为卵巢癌治疗手段,提供了理论与实验依据。To study the expression pattern of miRNA-200c in ovarian serous adenocarcinoma at stage Ⅲc, and to explore the possible role in tumor metastasis , we collected ovarian tumor tissue specimens from 2010 January to 2013 December in Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine, including 30 cases of ovarian serous cystadenoma as control group and 40 cases of Ⅲc ovarian serous adenocarcinoma （40 cases of primary lesions and 40 cases of self-paired metastatic lesions） to detect the miRNA-200c expression by Real-time qPCR. Cell proliferation, migration and invasion ability after the ceil overexpressed miRNA-200c by the method of CCK8 and Transwell respectively. Detect the related protein quantity by Western Blot. We found （1） compared with the primary tumor in situ, miRNA-200c expression at stage Ⅲc ovarian serous adenocarcinoma metastatic tumors was elevated （490.37±78.32 cf. 157.46±17.21）, P〈0.01; these were both higher than its expression in ovarian serous cystadenoma （25.32± 10.17）, P〈0.01. （2） The migration ability of SKOV3 cells decreased afteroverexpressed of miRNA-200c （35.6±4.3 cf. 18.6±1.9）, P〈0.05; and the cell invasive capacity also reduced （26.7±3.1 cf. 15.4±2.4） P〈0.05; but the cell proliferation ability didn＇t change. （3） In protein level, the ZEB1 expression decreased after overexpressed miRNA-200c （0. 3023±0. 0251 cf. 0. 6753±0. 0262）, with E-cad protein expression elevated （1. 274± 0. 0331 cf. 0. 6140±0. 0460） and Vimentin protein expression decreased （0. 0957±0. 0049 cf. 0. 1767±0. 0158） P〈0.05. So miRNA-200c expression significantly increased in the tissues derived from the advanced and metastasis ovarian serous carcinoma patients, which indicated that miRNA-200c might act as a potential biomarker in evaluation of the advanced ovarian serous carcinoma metastasis process and development.

关 键 词：卵巢浆液性囊腺癌 晚期 MIR-200C 转移灶 

分 类 号：R737[医药卫生—肿瘤]