黑色素瘤组织中RI的表达量检测及过表达RI对B16-F10细胞体内外生长的调节作用  

Expression of ribonuclease inhibitor in melanoma tissue and the regulating effects of overexpressed ribonuclease inhibitor on B16-F10 cell growth in vivo and in vitro

作　　者：吴远慧[1] 黄攀登[1] 安国芝[1] 周向昭[1] 

机构地区：[1]河北北方学院附属第一医院皮肤科,河北张家口075000

出　　处：《海南医学院学报》2016年第4期313-315,319,共4页Journal of Hainan Medical University

基　　金：河北省高等学校科学技术研究项目(Q2012880)~~

摘　　要：目的:研究黑色素瘤组织中核糖核酸酶抑制因子(RI)的表达量及过表达RI对B16-F10细胞体内外生长的调节作用。方法:取黑色素瘤组织和肿瘤旁正常组织,检测RI的表达量;培养B16-F10细胞,过表达RI后测定细胞的增殖、侵袭行为以及相关基因的表达量;建立黑色素瘤移植瘤动物模型,称量移植瘤的重量。结果:(1)临床组织标本相关结果:不同TNM分期黑色素瘤组织中RI的表达量均低于肿瘤旁正常组织且TNM分期越高、RI的表达量越低;(2)细胞实验相关结果:过表达RI组细胞的OD值显著低于阴性对照组,发生侵袭的细胞数目少于阴性对照组,PKB、GSK-3β、ILK、PI3K、AKT的mRNA含量显著低于阴性对照组;(3)动物实验相关结果:过表达RI组小鼠的黑色素瘤移植瘤重量低于阴性对照组。结论:黑色素瘤组织中RI的表达量显著减少,增加RI的表达能够抑制黑色素瘤细胞的体内外生长。Objective： To investigate the expression of ribonuclease inhibitor （RI） in melanoma tissue and the regulating effects of the over-expressed RI on B16-F10 cell growth in vivo and in vitro. Methods： Melanoma tissue and adjacent normal tissue were collected to detect RI expression levels~ B16-F10 cells were cultured and after overexpression of RI, cell prolifera- tion and invasion as well as related gene expression levels were detected~ animal models with melanoma xenografts were built and weight of xenografts was weighed. Results： （1） Clinical tissue sample-related results： RI expression levels in melanoma tissue with different TNM stages were lower than those in adjacent normal tissue, and the higher the TNM stage, the lower the RI expression levels were; （2） cell experiment-related results： OD value of overexpressed RI group was significantly lower than that of negative control group, the number of invasive cells was less than that of negative control group, and mRNA con- tents of PKB, GSK-313, ILK, PI3K and AKT were significantly lower than those of negative control group;（3） Animal experi ment-related results ： weight of melanoma xenografts of overexpression RI group was lower than that of negative control group. Conclusion： RI expression significantly decreases in melanoma tissue, and increasing RI expression can inhibit melanoma cell growth in vivo and in vitro.

关 键 词：黑色素瘤 核糖核酸酶抑制因子(RI) 侵袭 增殖 移植瘤小鼠 

分 类 号：R739.5[医药卫生—肿瘤]