3种多环芳烃对条纹锯鮨胚胎发育及早期仔鱼的毒性效应  

Toxic effects of three polycyclic aromatic hydrocarbons on early embryonic and larval development of Centropristis striata

作　　者：孔祥迪[1,2] 刘莉[1,2] 李炎璐[2] 于欢欢[1,2] 陈超[2] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《中国水产科学》2016年第1期241-249,共9页Journal of Fishery Sciences of China

基　　金：科技部国际合作项目(2012DFA30360);国家科技支撑项目(2011BAD13B01)

摘　　要：为探讨不同多环芳烃(PAHs)对海水鱼类早期生长发育的毒性效应及作用机制,分别比较研究了多环芳烃中的3环菲(Phe)、4环芘(Py)、5环苯并芘(Bap)单一暴露和与α-萘黄酮(ANF)联合暴露对条纹锯鮨(Centropristis striata)胚胎发育及早期仔鱼的毒性效应。对其胚胎中的细胞色素酶(EROD)活性、发育畸形(以心脏畸形为准)、孵化率和早期仔鱼死亡率等指标进行测定分析,结果显示:(1)Bap、Py的浓度与EROD活性之间的剂量效应关系比Phe明显,3种PAHs对条纹锯鮨胚胎中EROD活性的诱导能力由高到低依次为Bap、Py、Phe,其活性的最大值均出现在各物质的最高浓度组,其活性分别为对照组的857.52%、514.21%、280.50%;在ANF影响下,EROD的活性诱导被抑制,最高浓度组的活性分别为对照组的189.27%、278.55%、195.40%。(2)综合考虑孵化率、畸形指数和早期仔鱼死亡率等指标,3种PAHs对胚胎发育及早期仔鱼的毒性由高到低依次为Bap、Py、Phe,3种物质的浓度与受精卵的孵化率呈明显负相关,与畸形指数和仔鱼死亡率呈明显正相关;在ANF影响下,Bap和Py处理组的毒性效应得到明显抑制,Phe与ANF联合暴露处理组的毒性效应与单一暴露处理相比无相关性(P>0.05)。研究表明,3种PAHs对条纹锯鮨的作用机制存在一定差异,Phe为非特异性麻醉毒性机制,Bap和Py可能通过AHR途径和CYP1 A活性诱导两种机制共同作用。We conducted this study to determine the toxicity mechanism of polycyclic aromatic hydrocarbons （PAHs） on the early development of the marine fish Centropristis striata. We analyzed individual exposure to phenanthrene （Phe）, pyrene （Py）, and benzo（a）pyrene （BaP） and co-exposure of each with u-naphthoflavone （ANF） to assess their toxic effects on early embryonic and larval development of C. striata. The results showed a clear dose-response relationship between Bap and Py concentration and ethoxyresorufin-O-deethylase （EROD） activ- ity compared with that of Phe. The effects of the PAHs on EROD activity were in the order Bap （857.52% of con- trol） 〉 Py （514.21%） 〉 Phe （280.50%）. Maximum EROD activity occurred at the highest concentration compared with that of the control group. EROD activity was inhibited （189.27%, 278.55%, and 195.40% of the control） when ANF was added to Bap, Py, and Phe, respectively. Toxicity of the PAHs to hatching rate, deformity index, and early embryo and larval mortality was in the order of Bap 〉 Py 〉 Phe. PAH concentration and the percentage of fertilized eggs and hatching rate were negatively correlated but PAH concentration was positively corre- lated with larval mortality and the deformity index. Bap and Py showed reduced toxicity when combined with ANF. These results show that PAHs with different ring structures have obvious toxic effects, although there were differences in the mechanisms among them.

关 键 词：条纹锯鮨 多环芳烃 胚胎 仔鱼 毒性效应 

分 类 号：X171[环境科学与工程—环境科学]