盐渍化土壤翅碱蓬根际促生细菌的筛选及对多环芳烃的降解特性  

Screening of a plant growth-promoting rhizobacterium from Suaeda salsa( L.) in saline soil and its ability to degrade polycyclic aromatic hydrocarbons

作　　者：宋立超[1] 钮旭光[1] 张玉龙[1] 刘宛[2] 李培军[2] 

机构地区：[1]沈阳农业大学土地与环境学院,沈阳110866 [2]中国科学院沈阳应用生态研究所,沈阳110016

出　　处：《生态学杂志》2016年第1期199-204,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(31401322);沈阳农业大学校青年基金项目(20111006)资助

摘　　要：为了强化多环芳烃污染盐渍化土壤的原位植物-微生物修复的应用,获得促进植物降解多环芳烃的高效菌种资源,本研究采用富集培养的方法,从多环芳烃污染的大港油田翅碱蓬根际分离到1株能以菲、芘为唯一碳源同时分泌1-氨基环丙烷^(-1)-脱氨酶的优势菌株B^(-1),通过形态观察和16S rRNA序列分析鉴定该菌株,并对其潜在的促生能力和菲、芘的降解特性进行分析。16S rRNA序列分析结果表明,该菌株为动性球菌属(Planococcus sp.),可产生3-吲哚乙酸,且具有溶磷能力。同时对菲、芘的降解具有较广泛的p H值和盐浓度范围,在菲和芘浓度均为50 mg·L^(-1),p H 8.0,盐度2%时,7 d对菲、芘的降解率为66.6%和52.0%。表面活性剂烷基糖苷的添加使菲、芘降解效率分别提高至94.2%和78.8%。In order to apply the microbial-phytoremediation for the treatment of saline soil con- taminated by polycyclic aromatic hydrocarbons （PAHs） , the PAHs-specific degrading bacteria were studied in this paper. Phenanthrene and pyrene were used as carbon and energy sources, and one dominant strain （B-I） producing 1-aminocyclopropane-l-carboxylate （ACC） deaminase was isolated by enrichment culture from the PAHs-contaminated soil at Dagang Oilfield. The mor- phology and 16S rRNA gene sequencing analysis revealed that this strain belonged to Planococcus sp., and it could produce indol-3-ylacetic acid and dissolve phosphates. With phenanthrene and cantly increased to 94.2% and 78.8% when alkyl polyglycoside was added into the system

关 键 词：促生菌 动性球菌属 土壤污染 盐渍化 

分 类 号：X172[环境科学与工程—环境科学] X53