STAT3,pSTAT3与TFF3在甲状腺乳头状癌中的临床意义  

Clinical significance of STAT3, pSTAT3 and TFF3 in human papillary thyroid carcinoma

作　　者：张晨蕾 李燕萍[2] 吴靖芳[3] 万佳鑫 陈亚敏[1] 张文静[3] 薛刚[2] 

机构地区：[1]河北北方学院基础医学院,本科生河北张家口075000 [2]河北北方学院附属第一医院耳鼻咽喉头颈外科,河北张家口075000 [3]河北北方学院基础医学院组胚教研室,河北张家口075000

出　　处：《现代预防医学》2016年第1期182-186,共5页Modern Preventive Medicine

基　　金：河北省重大医学科研课题(ZD2013052);河北北方学院重大项目和校级课题(ZD20130015,2014011)

摘　　要：目的探讨甲状腺乳头状癌（Papillary Thyroid Carcinomas,PTC）STAT3及其磷酸化水平与三叶因子3（Trefoil Factor 3,TFF3）表达及相关性。方法采用免疫组织化学检测31例组织芯片中PTC及癌旁组织STAT3、p STAT3和TFF3阳性率与平均光密度（AOD）值;Western Blot检测6例PTC和癌旁手术标本中相应蛋白的含量,并进行相关分析。结果 （1）免疫组化：TFF3蛋白在PTC中均高表达（P〈0.001）,TFF3阳性率与AOD值在直径〉2 cm组、淋巴结转移组和临床Ⅲ-Ⅳ病例明显高于直径≤2 cm组、无淋巴结转移组和Ⅰ-Ⅱ期者（χ^2=12.120,P=0.001,χ^2=7.517,P=0.043,χ^2=10.490,P=0.003;AOD值：t=2.810,P=0.0064,t=2.234,P=0.039,t=2.926,P=0.0058）;PTC中STAT3阳性率和AOD值与临床资料无关。p STAT3为胞核阳性,阳性率和AOD值在PTC组织低于癌旁组织,直径〉2 cm组、淋巴结转移组和临床Ⅲ-Ⅳ病例明显低于直径≤2 cm组、无淋巴结转移组和Ⅰ-Ⅱ期者（χ-2=4.963,P=0.046,χ-2=4.882,P=0.033;AOD值：t=2.195,P=0.041,t=2.790,P=0.0068,t=2.018,P=0.042）;TFF3与STAT3呈正相关,与p STAT3呈负相关（P〈0.01）。（2）Western Blot：TFF3和STAT3蛋白在PTC的表达高于癌旁组织（t=2.841,P=0.008,t=2.925,P=0.007）,但p STAT3却明显低于癌旁组织（t=2.713,P=0.009）。结论甲状腺乳头状癌TFF3和STAT3高表达、p STAT3低表达对PTC不良预后的预测有指导意义。Objective To investigate the expression and correlation of STAT3, p STAT3 with TFF3 in the papillary thyroid carcinomas（PTC）. Methods Immunohistochemistry was used to investigate the positive expression and the average optical density（AOD） value of STAT3, p STAT3 and TFF3 among 31 cases PTC and para-carcinoma tissue chips. Western blot was used to detect the protein content among 6 cases PTC and para-carcinoma specimens. Results（1） Immunohistochemistry： TFF3 was highly expressed in PTC. It＇s positive rate and AOD value were higher in diameter 2cm, lymph node metastasis group and clinical Ⅲ-Ⅳstage cases than those of diameter ≤2cm group, without lymph node metastasis group and Ⅰ-Ⅱstage（χ^2=12.120, P=0.001,χ^2=7.517, P=0.043, χ^2=10.490, P=0.003; AOD value： t=2.810, P=0.0064, t=2.234, P=0.039, t=2.926, P=0.0058）. The positive rate and AOD value of STAT3 had nothing to do with clinical data. p STAT3 mainly expressed in nucleus, whose positive rate and AOD value were lower in para-carcinoma. The positive rates of diameter 2cm group, lymph node metastasis group and clinical Ⅲ-Ⅳstage cases were lower than those of diameter ≤2cm group, without lymph node metastasis group andⅠ-Ⅱstage cases（χ^2=4.963,P=0.046, χ^2=4.882, P=0.033; AOD value： t=2.195, P=0.041, t=2.790, P=0.007, t=2.018, P=0.042）. A positive relationship between TFF3 with STAT3, and an inverse relationship between TFF3 with p STAT3 were observed（P0.01）.（2） Western blot： The content of TFF3 and STAT3 protein in PTC were higher than para-carcinoma tissue（t=2.841, P=0.008, t=2.925, P=0.007）, but p STAT3 was lower than para-carcinoma（t=2.713, P=0.009）. Conclusion There may be a guiding significance for poor prognosis prediction of PTC when TFF3 and STAT3 high expressed with low expression of p STAT3 in PTC tissue.

关 键 词：三叶因子3(Trefoil Factor 3 TFF3) 信号转导和转录激活因子(Signal Transducers and ACTIVATORS of Transcription 3 STAT3) 乳头状甲状腺癌 免疫组化 蛋白印迹(Western Blot) 

分 类 号：R113[医药卫生—公共卫生与预防医学]