喉癌患者血浆中miRNA表达及基于靶基因的能量消耗的研究  

Plasma-based miRNA Target Gene Expression and Energy Consumption in Laryngeal Cancer

作　　者：吴硕[1] 牛健[1] 李丽[1] 刘冰[1] 闫加兴[1] 曲学华[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,150001

出　　处：《实用癌症杂志》2016年第1期18-21,共4页The Practical Journal of Cancer

基　　金：黑龙江省自然基金立项课题(编号:D201068)

摘　　要：目的研究喉癌患者血浆中miRNA表达及基于靶基因的能量消耗。方法收集5例喉癌患者的病理组织。空腹抽血,分离人喉癌细胞系Hep2,从HEp2细胞中提取所有RNA。同时采用食物频率问卷(FFQ)收集患者日常饮食频率。同时进行营养生物标志物研究,记录4天的饮食记录(4DFR)进行饮食调整(DM)。结果实时PCR结果显示,与无转染的喉癌细胞比较,在pc DNA3/Pri-miR-1转染的HEp2喉癌细胞中miR-1水平升高3.8倍。但是在miR-1ASO转染的HEp2细胞中miR-1下降了70%。miR-1能够促进HEp2细胞集落形成,但不影响细胞生存力。患者能量消耗研究发现,与对照患者比较,脂肪和总能量消耗增加的风险因素是喉癌患者的身体质量指数和年龄。结论研究表明,miRNA可改变癌症细胞中的脂质和氨基酸代谢;miRNA可以成为治疗癌症的新靶点。Objective To study the plasma-based miRNA target gene expression and energy consumption in laryngeal cancer. Methods Pathology tissues of 5 cases of laryngeal cancer were collected. Fasting blood,isolated from human laryngeal carcinoma cell line Hep2,all RNA were extracted from HEp2 cells. Food frequency questionnaire（ FFQ） was used to collect the frequency of patients daily diet. Meanwhile nutritional biomarker studies,4-day diet records（ 4DFR） were recorded to conduct dietary adjustments（ DM）. Results Real-time PCR results showed that compared to non-transfected cells larynx in pcDNA3 / PrimiR-1 transfected HEp2 laryngeal cancer cells,miR-1 levels increased by about 3. 8 times. However,miR-1 reduced about 70%in miR-1 ASO transfected HEp2 cells. miR-1 could promote HEp2 cell colony formation,but did not affect cell viability. Energy consumption of patients showed that compared to control patients,the total energy consumption of fat and increased risk factors for laryngeal cancer patients were body mass index and age. Conclusion miRNA can change lipid and amino acid metabolism of cancer cells; and miRNA may become a new target for cancer therapy.

关 键 词：喉癌 miRNA 能量消耗 风险比 临床治疗 

分 类 号：R739.65[医药卫生—肿瘤]